Intercellular permeation and cyclosis-mediated transport of a fluorescent probe in Characeae

Intercellular communication and transport is the essential prerequisite for the function of multicellular organisms. Simple diffusion as a transport mechanism is often inefficient in sustaining the effective exchange of metabolites, and other active transport mechanisms become involved. In this paper, we use the giant cells of characean algae as a model system to explore the role of advection and diffusion in intercellular transport. Using fluorescent dye as a tracer, we study the kinetics of the permeation of the fluorophore through the plasmodesmata complex in the node of tandem cells and its further distribution across the cell. To explore the role of cytoplasmic streaming and the nodal cell complex in the transport mechanism, we modulate the cytoplasmic streaming using action potential to separate the diffusive permeation from the advective contribution. The results imply that the plasmodesmal transport of fluorescent probe through the central and peripheral cells of the nodal complex is differentially regulated by a physiological signal, the action potential. The passage of the probe through the central cells of the nodal complex ceases transiently after elicitation of the action potential in the internodal cell, whereas the passage through the peripheral cells of the node was retained. A diffusion-advection model is developed to describe the transport kinetics and extract the permeability of the node-internode cell wall from experimental data.

Automated summary

In this study, the role of advection and diffusion in intercellular transport was investigated using giant cells of characean algae as a model system. By utilizing fluorescent dye as a tracer, the permeation kinetics of the fluorophore through the plasmodesmata complex in the node of tandem cells and its distribution across the cell were examined. The results demonstrated that the plasmodesmal transport of the fluorescent probe through the central and peripheral cells of the nodal complex is differentially regulated by the physiological signal, the action potential.