4.5 Article

Lipid packing is disrupted in copolymeric nanodiscs compared with intact membranes

Related references

Note: Only part of the references are listed.
Article Biology

Allosteric modulation of the adenosine A2A receptor by cholesterol

Shuya Kate Huang et al.

Summary: Cholesterol indirectly modulates the conformational equilibria and signaling of the adenosine A(2A) receptor by influencing membrane properties.

ELIFE (2022)

Article Multidisciplinary Sciences

Structural and mechanistic analysis of a tripartite ATP-independent periplasmic TRAP transporter

Martin F. Peter et al.

Summary: Tripartite ATP-independent periplasmic (TRAP) transporters are widely found in bacteria and archaea. The structure of the sialic acid TRAP transporter HiSiaQM was studied using cryo-EM and various biophysical techniques. The TRAP transporter is composed of a soluble substrate-binding protein (P-domain) and two transmembrane domains (Q- and M-domains), with the Q-domain enabling the formation of a monomeric elevator architecture. Single-molecule total internal reflection fluorescence (TIRF) microscopy and surface plasmon resonance were used to study the formation of the tripartite complex and investigate the impact of interface mutants. Additionally, high-affinity VHH antibodies were characterized for their ability to inhibit sialic acid uptake by binding to the periplasmic side of HiSiaQM, providing insights into TRAP transporter function inhibition in vivo.

NATURE COMMUNICATIONS (2022)

Review Biochemistry & Molecular Biology

Structures and Dynamics of Native-State Transmembrane Protein Targets and Bound Lipids

Michael Overduin et al.

Summary: Membrane proteins play essential roles in biological structures within asymmetric lipid bilayers. The use of native nanodiscs formed with specific copolymers allows for extraction of these proteins directly from diverse cell and organelle membranes. Understanding the interactions between transmembrane targets and lipids is crucial for the development of next-generation native nanodiscs with therapeutic applications.

MEMBRANES (2021)

Review Chemistry, Multidisciplinary

LAURDAN since Weber: The Quest for Visualizing Membrane Heterogeneity

German Gunther et al.

Summary: Chemists studying membrane bilayer heterogeneity may encounter phase segregation in artificial bilayers and small sterol and sphingolipid-enriched dynamic domains in biological bilayers. Techniques using fluorescent probes and microscopy can assess membrane heterogeneity at different temporal and spatial levels, providing valuable information about the physical-chemical aspects of the membrane.

ACCOUNTS OF CHEMICAL RESEARCH (2021)

Article Biochemistry & Molecular Biology

A 10-year meta-analysis of membrane protein structural biology: Detergents, membrane mimetics, and structure determination techniques

Brendon C. Choy et al.

Summary: The study analyzes all uniquely solved membrane protein structures from 2010 to 2019, revealing the significant impact of single-particle cryo-electron microscopy on the field and the unique detergent/membrane mimetic requirements of different structure determination methods and protein classes.

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES (2021)

Article Biology

Lipidomic and in-gel analysis of maleic acid co-polymer nanodiscs reveals differences in composition of solubilized membranes

Marta Barniol-Xicota et al.

Summary: The study found that maleic acid based copolymers can disrupt lipid bilayers and form lipid protein nanodiscs soluble in aqueous buffer. The content of xMALPs varies depending on the chemical properties of the used xMA.

COMMUNICATIONS BIOLOGY (2021)

Article Cell Biology

Plasma Membrane Fluidity: An Environment Thermal Detector in Plants

Dora L. Cano-Ramirez et al.

Summary: The lipid matrix in cell membranes is dynamic and plays a role in membrane fluidity changes in response to temperature fluctuation. The plasma membrane (PM) of plants is highly fluid and sensitive to temperature variations, serving as an ideal temperature detector with the ability to quickly adjust in response to small temperature changes. This makes the PM an optimal alternative for temperature detection in plants.

CELLS (2021)

Article Chemistry, Multidisciplinary

Understanding the Structural Pathways for Lipid Nanodisc Formation: How Styrene Maleic Acid Copolymers Induce Membrane Fracture and Disc Formation

Victoria Ariel Bjornestad et al.

Summary: Lipid nanodiscs formed by mixtures of styrene maleic acid (SMA) copolymers and lipid membranes are important tools for studying membrane proteins, with the formation pathways depending strongly on factors such as temperature, lipid/ copolymer ratio, and lipid type. The initial aggregation of copolymer in solution leads to insertion of its styrene units into the lipid bilayer hydrocarbon region, causing fractures in the membrane.

LANGMUIR (2021)

Article Biology

Membrane transporter dimerization driven by differential lipid solvation energetics of dissociated and associated states

Rahul Chadda et al.

Summary: The study reveals how the lipid solvent defines the dimerization equilibrium of membrane proteins, with monomers associating to avoid membrane defects caused by hydrophobic mismatch. Dimerization is driven by the larger free-energy cost of lipid solvation, and the addition of short-chain lipids can shift the equilibrium towards the monomeric state. This preferential lipid solvation mechanism may regulate oligomerization processes at low concentrations without global changes in membrane properties.

ELIFE (2021)

Article Biochemistry & Molecular Biology

Detergent-free purification and reconstitution of functional human serotonin transporter (SERT) using diisobutylene maleic acid (DIBMA) copolymer

Marvin Dilworth et al.

Summary: Despite the fundamental importance of human membrane proteins in cellular function and drug targeting, there is a lack of structure and function analysis in lipid bilayer environments. The traditional detergent purification method for studying these proteins is often destabilizing, leading researchers to explore alternative methods like the SMA extraction approach. This study successfully purified and analyzed the human serotonin transporter (hSERT) using a detergent-free method, showing improved thermostability and activity over conventional methods.

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES (2021)

Article Biochemistry & Molecular Biology

Evaluation of DIBMA nanoparticles of variable size and anionic lipid content as tools for the structural and functional study of membrane proteins

Natalia Voskoboynikova et al.

Summary: Amphiphilic maleic acid-containing polymers allow for the direct extraction of membrane proteins into stable, homogenous, water-soluble copolymer/lipid nanoparticles without the use of detergents. However, an increase in the size of the lipid nanoparticles may correlate with increased sample heterogeneity, thus hampering their application to spectroscopic and structural techniques where highly homogeneous samples are desirable.

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES (2021)

Article Biophysics

Functional integrity of membrane protein rhodopsin solubilized by styrene-maleic acid copolymer

Stephanie G. Pitch et al.

Summary: The study investigated the functional properties of the membrane protein rhodopsin in its native lipid environment after being solubilized with styrene-maleic acid (SMA) copolymer. Results showed that SMA is a useful tool for membrane protein research, but excessive addition of SMA can interfere with the dynamics of protein activation.

BIOPHYSICAL JOURNAL (2021)

Article Biophysics

Styrene-maleic acid copolymer effects on thefunction of the GPCR rhodopsin in lipidnanoparticles

Istvan Szundi et al.

Summary: Styrene-maleic acid (SMA) copolymers solubilize biological membranes to form nanoparticles containing membrane proteins surrounded by native lipids. Lower SMA/protein molar ratios are suitable for photokinetic studies, while higher ratios may lead to inaccurate results.

BIOPHYSICAL JOURNAL (2021)

Article Biochemistry & Molecular Biology

Structural biology of endogenous membrane protein assemblies in native nanodiscs

Chanelle J. Brown et al.

Summary: The development of amphiphilic copolymers has allowed the solubilization of integral membrane proteins into native nanodiscs, providing insights into their structures and functions. This breakthrough has established biological membrane:protein assemblies as fundamental units of cellular membranes, and various methods including cryo-EM and NMR spectroscopy have been utilized for the characterization of transmembrane proteins within these nanodiscs.

CURRENT OPINION IN STRUCTURAL BIOLOGY (2021)

Review Biochemistry & Molecular Biology

Insights into the Role of Membrane Lipids in the Structure, Function and Regulation of Integral Membrane Proteins

Kenta Renard et al.

Summary: Membrane proteins interact with membranes are crucial for cellular function, and recent advances in experimental methods have provided insight into the importance of lipids in maintaining the conformational state and activity of membrane proteins.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

Article Biochemistry & Molecular Biology

Purification of active human vacuolar H+-ATPase in native lipid-containing nanodiscs

Rebecca A. Oot et al.

Summary: V-ATPases are large proton pumps found in virtually every eukaryotic cell, with some isoforms of the a subunit playing a role in tissue-specific diseases and cancer progression. Purification of active human V-ATPases is challenging due to isoform complexity and low abundance, but a strategy involving lipid nanodiscs has been developed for isolation and analysis of the enzyme.

JOURNAL OF BIOLOGICAL CHEMISTRY (2021)

Article Cell Biology

Nano-scale resolution of native retinal rod disk membranes reveals differences in lipid composition

Christopher L. Sander et al.

Summary: The study revealed differences in lipid composition between the central and rim regions of photoreceptor ROS, highlighting potential protein-lipid interactions. High-resolution profiling of ROS disk lipid composition provides insights into the balance of intricate membrane structure and protein activity within the ROS, serving as a model for future studies of complex cellular structures.

JOURNAL OF CELL BIOLOGY (2021)

Article Multidisciplinary Sciences

Structure and function at the lipid-protein interface of a pentameric ligand-gated ion channel

Pramod Kumar et al.

Summary: The study focused on characterizing natively bound lipids in the superfamily of pentameric ligand-gated ion channels (pLGICs) using cryogenic electron microscopy. It identified two types of tightly bound lipids at the protein-bulk-membrane interface, shedding light on the interaction between membrane proteins and lipids.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2021)

Article Biochemistry & Molecular Biology

PIP2 promotes conformation-specific dimerization of the EphA2 membrane region

Katherine M. Stefanski et al.

Summary: The impact of EphA2 receptor on cancer malignancy is determined by two different activation methods. PIP2 signaling lipid promotes TM dimerization in a conformation resembling the ligand-independent state, potentially affecting EphA2 activation. The difference in TM crossing angles in the ligand-dependent and ligand-independent states may play a role in regulating EphA2 activity.

JOURNAL OF BIOLOGICAL CHEMISTRY (2021)

Article Chemistry, Physical

Lipidomic atlas of mammalian cell membranes reveals hierarchical variation induced by culture conditions, subcellular membranes, and cell lineages

Jessica L. Symons et al.

Summary: Lipid membranes are essential for the structural and functional compartmentalization of cells and sub-cellular organelles. This study used shotgun lipidomics to analyze the lipidomes of various cultured and primary mammalian membrane preparations, identifying clear differences between cultured and primary cells, as well as distinct lipidomic signatures of individual cell lines and tissue types. These findings contribute to a comprehensive understanding of mammalian lipidomes and their regulation in various contexts.

SOFT MATTER (2021)

Article Biochemistry & Molecular Biology

Factors influencing the solubilization of membrane proteins from Escherichia coli membranes by styrene-maleic acid copolymers

Adrian H. Kopf et al.

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES (2020)

Review Cell Biology

Lipid Rafts: Controversies Resolved, Mysteries Remain

Ilya Levental et al.

TRENDS IN CELL BIOLOGY (2020)

Article Biochemistry & Molecular Biology

Lipid dynamics in nanoparticles formed by maleic acid-containing copolymers: EPR spectroscopy and molecular dynamics simulations

Alexandr Colbasevici et al.

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES (2020)

Article Biochemical Research Methods

Lipid exchange among polymer-encapsulated nanodiscs by time-resolved Forster resonance energy transfer

Bartholomaus Danielczak et al.

METHODS (2020)

Article Biochemistry & Molecular Biology

Plasma membranes are asymmetric in lipid unsaturation, packing and protein shape

J. H. Lorent et al.

NATURE CHEMICAL BIOLOGY (2020)

Article Biochemistry & Molecular Biology

A comparison of SMA (styrene maleic acid) and DIBMA (di-isobutylene maleic acid) for membrane protein purification

Aiman A. Gulamhussein et al.

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES (2020)

Article Biophysics

Induction of Ordered Lipid Raft Domain Formation by Loss of Lipid Asymmetry

Johnna Wellman St Clair et al.

BIOPHYSICAL JOURNAL (2020)

Article Cell Biology

Liquid-Ordered Phase Formation by Mammalian and Yeast Sterols: A Common Feature With Organizational Differences

Alena Khmelinskaia et al.

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY (2020)

Article Chemistry, Analytical

Redesigning Solvatochromic Probe Laurdan for Imaging Lipid Order Selectively in Cell Plasma Membranes

Dmytro Danylchuk et al.

ANALYTICAL CHEMISTRY (2020)

Review Biochemistry & Molecular Biology

Current problems and future avenues in proteoliposome research

Andrea Marco Amati et al.

BIOCHEMICAL SOCIETY TRANSACTIONS (2020)

Article Biochemistry & Molecular Biology

Lipid Composition Affects the Efficiency in the Functional Reconstitution of the Cytochrome c Oxidase

Katharina Gloria Hugentobler et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020)

Article Multidisciplinary Sciences

Connexin-46/50 in a dynamic lipid environment resolved by CryoEM at 1.9 Å

Jonathan A. Flores et al.

NATURE COMMUNICATIONS (2020)

Review Cell Biology

Membrane Heterogeneity Beyond the Plasma Membrane

Hong-Yin Wang et al.

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY (2020)

Article Biochemistry & Molecular Biology

Complexity of seemingly simple lipid nanodiscs

Piotr Stepien et al.

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES (2020)

Review Biochemical Research Methods

Structures and Interactions of Transmembrane Targets in Native Nanodiscs

Michael Overduin et al.

SLAS DISCOVERY (2019)

Article Chemistry, Multidisciplinary

A fluorescent membrane tension probe

Adai Colom et al.

NATURE CHEMISTRY (2018)

Article Physics, Applied

Laurdan and Di-4-ANEPPDHQ probe different properties of the membrane

Mariana Amaro et al.

JOURNAL OF PHYSICS D-APPLIED PHYSICS (2017)

Article Biochemistry & Molecular Biology

Activation of the Unfolded Protein Response by Lipid Bilayer Stress

Kristina Halbleib et al.

MOLECULAR CELL (2017)

Article Multidisciplinary Sciences

Cholesterol-mediated allosteric regulation of the mitochondrial translocator protein structure

Garima Jaipuria et al.

NATURE COMMUNICATIONS (2017)

Article Multidisciplinary Sciences

Direct PIP2 binding mediates stable oligomer formation of the serotonin transporter

Andreas Anderluh et al.

NATURE COMMUNICATIONS (2017)

Article Multidisciplinary Sciences

Fast Collisional Lipid Transfer Among Polymer-Bounded Nanodiscs

Rodrigo Cuevas Arenas et al.

SCIENTIFIC REPORTS (2017)

Article Multidisciplinary Sciences

Thermodynamics of nanodisc formation mediated by styrene/maleic acid (2:1) copolymer

Anne Grethen et al.

SCIENTIFIC REPORTS (2017)

Article Biochemistry & Molecular Biology

Thermotropic properties of phosphatidylcholine nanodiscs bounded by styrene-maleic acid copolymers

J. J. Dominguez Pardo et al.

CHEMISTRY AND PHYSICS OF LIPIDS (2017)

Review Chemistry, Multidisciplinary

Nanodiscs in Membrane Biochemistry and Biophysics

Ilia G. Denisov et al.

CHEMICAL REVIEWS (2017)

Article Chemistry, Multidisciplinary

Solubilization of Membrane Proteins into Functional Lipid-Bilayer Nanodiscs Using a Diisobutylene/Maleic Acid Copolymer

Abraham Olusegun Oluwole et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2017)

Review Chemistry, Multidisciplinary

Solvatochromic and Fluorogenic Dyes as Environment-Sensitive Probes: Design and Biological Applications

Andrey S. Klymchenko

ACCOUNTS OF CHEMICAL RESEARCH (2017)

Article Biochemistry & Molecular Biology

The Lipid Bilayer Modulates the Structure and Function of an ATP-binding Cassette Exporter

Maria E. Zoghbi et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2016)

Article Biochemistry & Molecular Biology

A Eukaryotic Sensor for Membrane Lipid Saturation

Roberto Covino et al.

MOLECULAR CELL (2016)

Article Chemistry, Multidisciplinary

Influence of lipid bilayer properties on nanodisc formation mediated by styrene/maleic acid copolymers

Rodrigo Cuevas Arenas et al.

NANOSCALE (2016)

Article Biochemical Research Methods

A method for detergent-free isolation of membrane proteins in their local lipid environment

Sarah C. Lee et al.

NATURE PROTOCOLS (2016)

Article Biochemistry & Molecular Biology

Nanodiscs for structural and functional studies of membrane proteins

Ilia G. Denisov et al.

NATURE STRUCTURAL & MOLECULAR BIOLOGY (2016)

Article Biochemistry & Molecular Biology

Nanodiscs for structural and functional studies of membrane proteins

Ilia G. Denisov et al.

NATURE STRUCTURAL & MOLECULAR BIOLOGY (2016)

Article Multidisciplinary Sciences

Tissue-based map of the human proteome

Mathias Uhlen et al.

SCIENCE (2015)

Review Biotechnology & Applied Microbiology

Advances in the use of nanoscale bilayers to study membrane protein structure and function

Ketan Malhotra et al.

BIOTECHNOLOGY AND GENETIC ENGINEERING REVIEWS, VOL 30, ISSUE 1 (2014)

Article Multidisciplinary Sciences

Detergent-free isolation, characterization, and functional reconstitution of a tetrameric K+ channel: The power of native nanodiscs

Jonas M. Dorr et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2014)

Article Biochemical Research Methods

Biophysical characterization of membrane proteins in nanodiscs

Sayaka Inagaki et al.

METHODS (2013)

Article Chemistry, Multidisciplinary

Detergent-Free Formation and Physicochemical Characterization of Nanosized Lipid-Polymer Complexes: Lipodisq

Marcella C. Orwick et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2012)

Article Biophysics

Phase Separation and Fluctuations in Mixtures of a Saturated and an Unsaturated Phospholipid

James A. Svetlovics et al.

BIOPHYSICAL JOURNAL (2012)

Article Biochemical Research Methods

Prediction of the human membrane proteome

Linn Fagerberg et al.

PROTEOMICS (2010)

Review Cell Biology

Membrane lipids: where they are and how they behave

Gerrit van Meer et al.

NATURE REVIEWS MOLECULAR CELL BIOLOGY (2008)

Article Multidisciplinary Sciences

Protein area occupancy at the center of the red blood cell membrane

Allison D. Dupuy et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2008)

Article Biochemistry & Molecular Biology

A two-photon fluorescent probe for lipid raft imaging: C-laurdan

Hwan Myung Kim et al.

CHEMBIOCHEM (2007)

Article Biochemistry & Molecular Biology

Phospholipid phase transitions in homogeneous nanometer scale bilayer discs

AW Shaw et al.

FEBS LETTERS (2004)

Article Chemistry, Multidisciplinary

Self-assembly of discoidal phospholipid bilayer nanoparticles with membrane scaffold proteins

TH Bayburt et al.

NANO LETTERS (2002)