Journal
BIOELECTROCHEMISTRY
Volume 149, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2022.108312
Keywords
Impedance -based electrochemical sensor; Gold nanomaterials; Hybridization chain reaction (HCR); DNA self -assembly; Lead ion
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This study developed a label-free impedance-based electrochemical sensor for the quantitative detection of Pb2+. The use of conductive gold nanomaterials as electrode substrate materials provides sensors with a larger specific surface area, action sites, and excellent conductivity. DNA nanostructures were utilized for biomolecule determination, leading to successful quantitative detection of Pb2+. The designed sensors demonstrated good recoveries in real samples, making it highly applicable in various fields.
Here, a label-free impedance-based electrochemical sensor was developed for the quantitative detection of Pb2+. Using conductive gold nanomaterials as electrode substrate materials can provide sensors with larger specific surface area, action sites and excellent conductivity. DNA nanostructures are used for the determination of biomolecules due to their good properties. The Y-DNA structure is formed by the annealing of three DNA se-quences, which acts as a stable structure and forms a dendritic structure in combination with the hybrid chain reaction. In the presence of the target Pb2+, it induces the conversion of specific aptamers into G-quadruplexes, resulting in HCR and Y-DNA loading on the electrodes and a significant change in the impedance value signal. Therefore, the proposed biosensor realizes the quantitative detection of Pb2+. Under the optimal experimental conditions, the concentration of Pb2+ exhibited a linear correlation range from 0.5 to1000 nmol/L with a limit of detection (LOD) of 0.38 nmol/L. The designed sensors have good recoveries in real samples (tap water and tea). This flexible experimental protocol has broad application prospects.
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