Journal
JOURNAL OF NEUROSCIENCE
Volume 36, Issue 7, Pages 2161-2175Publisher
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.3181-15.2016
Keywords
memantine; neuroprotection; neurosteroid; NMDA receptor; pregnanolone sulfate; synaptic transmission
Categories
Funding
- Czech Science Foundation [303/12/1464, P304/12/G069, P208/12/G016]
- Technology Agency of the Czech Republic [TE01020028]
- Grant Agency of Charles University [1520-243-253483, 800313/2012/2]
- CAS [RVO: 67985823]
- Center of Biomedical Research by European Science Foundation [CZ.1.07/2.3.00/30.0025]
- Biotechnology and Biomedicine Center of the Academy of Sciences
- Charles University in Vestec by European Regional Development Fund [CZ.1.05/1.1.00/02.0109]
- Institute of Organic Chemistry and Biochemistry, CAS [RVO: 61388963]
- National Institute of General Medical Sciences [P41-GM103311]
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Postsynaptic N-methyl-D-aspartate receptors (NMDARs) phasically activated by presynaptically released glutamate are critical for synaptic transmission and plasticity. However, under pathological conditions, excessive activation of NMDARs by tonically increased ambient glutamate contributes to excitotoxicity associated with various acute and chronic neurological disorders. Here, using heterologously expressed GluN1/GluN2A and GluN1/GluN2B receptors and rat autaptic hippocampal microisland cultures, we show that pregnanolone sulfate inhibits NMDAR currents induced by a prolonged glutamate application with a higher potency than the NMDAR component of EPSCs. For synthetic pregnanolone derivatives substituted with a carboxylic acid moiety at the end of an aliphatic chain of varying length and attached to the steroid skeleton at C3, the difference in potency between tonic and phasic inhibition increased with the length of the residue. The steroid with the longest substituent, pregnanolone hemipimelate, had no effect on phasically activated receptors while inhibiting tonically activated receptors. In behavioral tests, pregnanolone hemipimelate showed neuroprotective activity without psychomimetic symptoms. These results provide insight into the influence of steroids on neuronal function and stress their potential use in the development of novel therapeutics with neuroprotective action.
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