Journal
JOURNAL OF NEUROSCIENCE
Volume 36, Issue 27, Pages 7325-7339Publisher
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.4282-15.2016
Keywords
dendritic protein synthesis; plasticity; retinotectal; visual avoidance behavior; visual conditioning; Xenopus
Categories
Funding
- National Institutes of Health Grant [EY011261]
- Salk National Eye Institute Core Grant P30 [EY019005]
- Hahn Family Foundation
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Fragile X mental retardation protein (FMRP) is thought to regulate neuronal plasticity by limiting dendritic protein synthesis, but direct demonstration of a requirement for FMRP control of local protein synthesis during behavioral plasticity is lacking. Here we tested whether FMRP knockdown in Xenopus optic tectum affects local protein synthesis in vivo and whether FMRP knockdown affects protein synthesis-dependent visual avoidance behavioral plasticity. We tagged newly synthesized proteins by incorporation of the noncanonical amino acid azidohomoalanine and visualized them with fluorescent noncanonical amino acid tagging (FUNCAT). Visual conditioning and FMRP knockdown produce similar increases in FUNCAT in tectal neuropil. Induction of visual conditioning-dependent behavioral plasticity occurs normally in FMRP knockdown animals, but plasticity degrades over 24 h. These results indicate that FMRP affects visual conditioning-induced local protein synthesis and is required to maintain the visual conditioning-induced behavioral plasticity.
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