Journal
CATALYSIS COMMUNICATIONS
Volume 66, Issue -, Pages 121-125Publisher
ELSEVIER
DOI: 10.1016/j.catcom.2015.03.035
Keywords
Biocatalysis; Gabapentin; Hydrogenation; Protein engineering; Regioselectivity
Categories
Funding
- Zhejiang Major International Science and Technology Cooperation Project [2013C24013]
- NSFC [31170761]
Ask authors/readers for more resources
An efficient chemoenzymatic process is devised for synthesizing high-purity gabapentin. 1-Cyanocyclohexaneacetic acid was first produced in 0.94 M from 1.0 M 1-cyanocycloalkaneacetonitrile by a greatly improved nitrilase from Acidovorax facilis ZJB09122, resulting in a commercially attractive bioprocess with an outstanding space-time yield of 461 g/L/day. The resulting aqueous 1-cyanocycloalkaneacetic acid was then directly converted to 2-azaspiro [4.5] decan-3-one without further purification in subsequent hydrogenation by Raney-nickel, followed by simple chemical steps to afford gabapentin in high purity and 77.3% overall yield from 1-cyanocyclohexylacetonitrile. The simplicity of the process makes this new pathway suitable for large-scale preparation. (c) 2015 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available