Identification and expression analyses of the olfactory-related genes in different tissues' transcriptome of a predacious soldier beetle, Podabrus annulatus (Coleoptera, Cantharidae)

We sequenced and analyzed the transcriptomes from different tissues of the soldier beetle, Podabrus annulatus (Coleoptera: Cantharidae), and obtained 75.74 Gb clean reads which were assembled into 95,274 unigenes. Among these transcripts, 25,484 unigenes of highly quality were annotated. Based on annotation and tBLASTn results, we identified a total of 101 candidate olfactory-related genes for the first time, including 11 putative odorant-binding proteins (OBPs), 6 chemosensory proteins (CSP), 50 olfactory receptors (ORs), 25 gustatory receptors (GRs), 6 ionotropic receptors (IRs), and 3 sensory neuron membrane proteins (SNMPs). BLASTX best-hit results indicated that these chemosensory genes were most identical to their respective orthologs from Photinus pyralis. Phylogenetic analyses also revealed that the ORs, GRs, and IRs of Podabrus annulatus are closely related to those of Photinus pyralis. The fragment per kilobase per million mapped fragments (FPKM) values showed that the PannOBP2, PannOBP3, and PannOBP10 were predominantly expressed in the antennae, PannOBP1 in the abdomen-thorax, while others were not identified to be tissue-specific. These olfactory-related differentially expressed genes (DEGs) demonstrated different roles in the olfactory system of Podabrus annulatus. This study establishes the groundwork for future research into the molecular mechanism of olfactory recognition in Podabrus annulatus.

Automated summary

In this study, transcriptomes from different tissues of the soldier beetle Podabrus annulatus were sequenced and analyzed. A total of 75.74 Gb clean reads were obtained and assembled into 95,274 unigenes. Annotation and tBLASTn results identified 101 candidate olfactory-related genes, including OBPs, CSPs, ORs, GRs, IRs, and SNMPs. Phylogenetic analyses showed a close relationship between Podabrus annulatus and Photinus pyralis in terms of ORs, GRs, and IRs. Differential expression analysis revealed tissue-specific expression patterns of certain OBPs. This study lays the foundation for future research on the molecular mechanism of olfactory recognition in Podabrus annulatus.