Journal
AQUACULTURE
Volume 561, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.aquaculture.2022.738650
Keywords
Mandarin fishes; Pool -sequencing; Sex markers; Sex chromosome; Sex differentiated region
Categories
Funding
- High-Level Talent Agglomeration Program of Hunan, China [2019RS1044]
- earmarked fund for Agriculture Research System of China [2019RS1044]
- National Natural Science Foundation of China [CARS-45, 32002372]
- Key Research and Development Program of Hunan Province [32002382]
- High-level Talent Agglomera-tion Program of Hunan, China [2019RS1044]
- Laboratory of Lingnan Modern Agriculture Project [2018NK2072]
- [NT2021008]
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In this study, we characterized sex-specific markers and sex determination in mandarin fishes. We found significant sex-differentiated regions and sex chromosomes in Siniperca chuatsi as well as other mandarin fishes. These non-recombining regions contained male-biased genetic polymorphisms and were conserved among the species. We successfully developed male-specific genetic markers and found a male heterogametic (XX-XY) sex determination system. Our study has important implications for future research and aquaculture applications in mandarin fishes.
Sex determination of teleost fish switched frequently. Sex markers are especially practical in identifying early sex and helpful in generating mono-sex population in aquatic species. And sex determination in mandarin fishes is still unclear. In this study, we applied a pool-sequencing approach to better characterize sex-specific markers and sex determination in mandarin fishes. Using genome comparative analysis, we identified significant sex differ-entiated region (-5.0 Mb) and sex chromosome (LG2) in Siniperca chuatsi. Similar sex-differentiated regions were also identified on LG4 in other mandarin fishes including Siniperca knerii, Siniperca scherzeri, and Coreoperca whiteheadi. These non-recombining regions contained high-density of male-biased genetic polymorphisms accompanied with male-biased fragments (-23 kb in total). Meanwhile, sequence synteny analysis showed that whole genomes, including sex chromosomes and even sex-differentiated regions among these species were in good synteny. In addition, the sex-differentiated region harbored 124 genes without usual suspects sex determining genes. Based on this genetic information, we successfully developed male-specific genetic markers for mandarin fishes. And genotyping results showed phenotypic sexes were strongly linked to its genetic sexes in one Siniperca chuatsi population. Our results exhibited that sex-differentiated regions and sex chromosomes were relatively conserved in the four mandarin fishes. All the four mandarin fishes were identified to utilize a male heterogametic (XX-XY) sex determination system. Besides, we also developed sex-linked genetic markers. Our study will facilitate future research on sex determination and aquaculture applications in mandarin fishes.
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