Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 107, Issue 1, Pages 247-260Publisher
SPRINGER
DOI: 10.1007/s00253-022-12285-3
Keywords
Shewanella hanedai; Arachidonate 9S-lipoxygenase; Characterization; C20-and C22-polyunsaturated fatty acids; Whole-cell conversion; Hydroxy fatty acids
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The putative lipoxygenase from Shewanella hanedai was identified as arachidonate 9S-lipoxygenase. Optimization of conditions led to increased production of C20 9S- and C22 11S-hydroxy fatty acids.
The putative lipoxygenase (LOX) from the proteobacterium Shewanella hanedai was determined to be an 82 kDa monomeric enzyme by SDS-PAGE and gel filtration chromatography analysis. LOX was identified as a single-dioxygenating arachidonate (ARA) 9S-LOX by analyzing ARA-derived bioconversion products using high-performance liquid chromatography with reverse-, normal-, and chiral-phase columns and evaluating kinetic parameters for C20- and C22-polyunsaturated fatty acids (PUFAs). The catalytic efficiency (k(cat)/K-m) values of 9S-LOX from S. hanedai for ARA, eicosapentaenoic acid, and docosahexaenoic acid were 3.1-, 4.1-, and 2.5-fold higher, respectively, than those only reported 9S-LOX from Sphingopyxis macrogoltabida with double-dioxygenating activity. To promote the production of C20 9S- and C22 11S-hydroxy fatty acids (HFAs) using Escherichia coli expressing 9S-LOX from S. hanedai, bioconversion conditions, including temperature, pH, solvent type and its concentration, concentrations of cells, and substrate, were optimized to 25 degrees C, pH 8.5, 6% (v/v) dimethyl sulfoxide, 0.2 g/l cells, and 7 mM ARA as substrate in a 500 ml-Erlenmeyer baffled flask with 50 ml reaction solution with agitation at 200 rpm in the presence of 10 mM cysteine as a reduction agent, respectively. Under these conditions, 6.4 mM 9S-hydroxyeicosatetraenoic acid, 6.2 mM 9S-hydroxyeicosapentaenoic acid, and 5.9 mM 11S-hydroxydocosahexaenoic acid were produced in 30 min, 40 min, and 60 min with specific productivities of 1067 mu mol/min/g, 775 mu mol/min/g, and 492 mu mol/min/g, volumetric productivities of 213 mu M/min, 155 mu M/min, and 98 mu M/min, and conversion yields of 91.4%, 88.6%, and 84.3%, respectively. To date, these are the highest specific productivities reported for the bioconversion of C20- and C22-PUFAs into HFAs. Graphical Abstract Key points Lipoxygenase from Shewanella hanedai was identified as arachidonate 9S-lipoxygenase Optimization led to increased production of C20 9S- and C22 11S-hydroxy fatty acids We reported the highest specific productivities of C20- and C22-hydroxy fatty acids
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