Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 195, Issue 5, Pages 2933-2946Publisher
SPRINGER
DOI: 10.1007/s12010-022-04236-5
Keywords
Molecular taxonomy; 16S rRNA; In silico secondary structure and restriction digestion; Phylogenetic tree; Spirulina; Curcumin; Calebin-A
Ask authors/readers for more resources
Due to morphological convergence and taxonomic concepts, the systematics of filamentous cyanobacteria remains a problem. This study evaluated the use of 16S rRNA gene sequences to infer genetic relationships among freshwater cyanobacterial isolates, and the taxonomic resolution was determined through phylogenetic analysis, restriction digestion, and structure prediction.
Due to morphological convergence and the application of numerous taxonomic concepts, the systematics of filamentous cyanobacteria is still a significant problem. The incorporation and integration of modern molecular, cyto-morphological and ecological approaches in cyanobacterial taxonomy are essential and must be acknowledged as the valid methods for the development of their modern systematics. In this study, method of using 16S rRNA gene sequences to infer the genetic relationships of twelve freshwater cyanobacterial isolates amongst themselves was evaluated. The taxonomic resolution was inferred from their phylogenetic tree, in silico restriction digestion analysis and secondary structure prediction. These methods allowed cyanobacterial genera to be well distinguished with their genotypic and phenotypic differences. Amongst twelve strains, Spirulina subsalsa with highest protein content was used in this study for evaluating the stability of Curcumin which is a curcuminoid compound reported from Curcuma longa. Though they have wide properties, they still lack stability and bioavailability. It is reported previously that microbes are used for biotransformation and act as a carrier molecule. Therefore, in this study, Spirulina incorporated with curcumin resulted with pH stability of curcumin and were found to have a biotransformation into Calebin-A, curcuminoid compound originally present in smaller amount (0.005%) in C. longa with various biomedical applications.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available