Ultrafast colorimetric detection of Cr(vi) using Fe3O4@polydopamine/Prussian blue composites as a highly efficient peroxidase mimic

A recyclable peroxidase mimic Fe3O4@polydopamine/Prussian blue (Fe3O4@PDA/PB) composite was facilely prepared by coating PDA on an Fe3O4 nanoparticle core and in situ growth of PB nanoparticles on a PDA shell. The prepared Fe3O4@PDA/PB composite exhibited excellent peroxidase-like activity and can catalytically oxidize the colorless colorimetric substrate 3,3 ',5,5 '-tetramethylbenzidine (TMB) into a blue colored product in the presence of H2O2 at 30 degrees C in 1 min. The catalytic mechanism was deduced to be the nanozyme-promoted generation of a hydroxyl radical (center dot OH), and the catalytic behavior followed the typical Michaelis-Menten kinetics. Based on Cr(vi)-boosted peroxidase-like activity of Fe3O4@PDA/PB, a simple and fast colorimetric method for detection of Cr(vi) was developed. Under the optimum conditions, the colorimetric method exhibited wider linear range (100 nM to 140 mu M), low LOD (51.1 nM), good selectivity and short detection time (1 min). Moreover, the feasibility of the proposed colorimetric method was evaluated by determination of Cr(vi) in spiked tap water and lake water samples. Good recoveries (95.2-102.9%) and low relative standard deviations (RSDs) (1.6-4.4%) were obtained, showing great promise for practical use.

Automated summary

A recyclable peroxidase mimic Fe3O4@polydopamine/Prussian blue composite was prepared and exhibited excellent peroxidase-like activity. The catalytic behavior followed typical Michaelis-Menten kinetics and the catalytic mechanism was deduced to be the nanozyme-promoted generation of a hydroxyl radical. A simple and fast colorimetric method for detection of Cr(vi) was developed based on the Cr(vi)-boosted peroxidase-like activity of Fe3O4@PDA/PB.