Journal
ANALYTICAL CHEMISTRY
Volume -, Issue -, Pages 1823-1828Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.2c04279
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Understanding diseases requires understanding metabolic heterogeneity in cells. This technical note introduces a 3D printing technology for manufacturing an ionization source for mass spectrometry-based single-cell analysis. The all in-one 3D-printed electrospray ionization source simplifies the process and improves reproducibility. It has been successfully used for high-throughput analysis of cancer cells and can be adjusted for different cell sizes. This 3D-printed ionization source opens new possibilities for single-cell analysis.
Understanding the physiologies and pathologies of diseases requires a thorough understanding of metabolic heterogeneity in cells. This technical note presents a 3D printing technology for manufacturing an ionization source that is specially adapted for mass spectrometry-based single-cell analysis. This all in-one 3D-printed electrospray ionization source integrates the sample introduction, metabolite extraction, and ionization into one device, simplifying the process of single-cell analysis and improving the reproducibility of the measurement. We successfully used it for high-throughput analysis of three types of cancer cells (around 17 cells/min) and used the t-distributed stochastic neighbor embedding algorithm to distinguish different cell types based on detected metabolites. By simply adjusting the printing parameters of the 3D-printed ionization source, it can be applied to cells with different sizes. The proposed 3D-printed ionization source promises to open new possibilities for single-cell analysis.
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