Journal
ANALYTICAL CHEMISTRY
Volume 94, Issue 51, Pages 17795-17802Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.2c03437
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Categories
Funding
- National Key Research and Development Program of China
- National Natural Science Foundation of China
- Science and Technology Planning Project of Fujian Province
- [2019YFD0901100]
- [21925404]
- [22022409]
- [22104124]
- [2019Y4001]
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Addressing the need for rapid, accurate, and low-cost diagnostic methods for detecting specific antigens for SARS-CoV-2 infection, a point-of-care SERS detection platform targeting the SARS-CoV-2 spike protein has been developed. The platform can specifically detect SARS-CoV-2 antigen in one step using aptamer-specific recognition, without the need for laboratory services or pretreatment. The detection can be completed within 5 minutes using a handheld Raman spectrometer.
Addressing the spread of coronavirus disease 2019 (COVID-19) has highlighted the need for rapid, accurate, and low-cost diagnostic methods that detect specific antigens for SARS-CoV-2 infection. Tests for COVID-19 are based on reverse transcription PCR (RT-PCR), which requires laboratory services and is time-consuming. Here, by targeting the SARS-CoV-2 spike protein, we present a point-of-care SERS detection platform that specifically detects SARS-CoV-2 antigen in one step by captureing substrates and detection probes based on aptamer-specific recognition. Using the pseudovirus, without any pretreatment, the SARS-CoV-2 virus and its variants were detected by a handheld Raman spectrometer within 5 min. The limit of detection (LoD) for the pseudovirus was 124 TU mu L-1 (18 fM spike protein), with a linear range of 250-10,000 TU mu L-1. Moreover, this assay can specifically recognize the SARS-CoV-2 antigen without cross reacting with specific antigens of other coronaviruses or influenza A. Therefore, the platform has great potential for application in rapid point-of-care diagnostic assays for SARS-CoV-2.
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