Journal
ANALYTICAL BIOCHEMISTRY
Volume 662, Issue -, Pages -Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2022.115014
Keywords
miRNA; EXPAR; Switchable nanomachine; Nb; BbvCI
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Sensitive and reliable quantitative determination of microRNA (miRNA) is crucial for disease diagnosis and cancer treatment. A novel miRNA detection approach based on target recognition initiated self-dissociation of DNA nanomachine is proposed. The method exhibits high sensitivity with a low detection limit of 12 fM and high selectivity that can distinguish one mismatched base pair. This approach is believed to be a robust tool for early cancer diagnosis and anticancer drug development.
As a valuable biomarker for various tumor, sensitive and reliable quantitative determination of microRNA (miRNA) is crucial for both disease diagnosis and cancer treatment. Herein, we depict a novel simple and sen-sitive miRNA detection approach by exploiting an elegantly designed target recognition initiated self-dissociation based DNA nanomachine. In this nanomachine, target recognition assists the formation of active DNAzyme secondary conformation, and the active DNAzyme generates a nicking site in H probe, initiating the self-assembly of H probe. With the reflexed sequences as primer, dual signal recycles are formed under the cooperation of DNA polymerase and Nb.BbvCI. Eventually, the method exhibits a high sensitivity with the limit of detection as low as 12 fM. In addition, the method is also demonstrated with a high selectivity that can distinguish one mismatched base pair. We believe the established approach can be a robust tool for the early-diagnosis of a variety of cancers and also in anticancer drug development.
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