Journal
ANALYTICAL BIOCHEMISTRY
Volume 658, Issue -, Pages -Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2022.114932
Keywords
Boar taint; Androstenone; Pheromones; MDGC-MS; SPE-Extraction; Sterol compounds
Funding
- Ministry of Agriculture of the Czech Republic [QK 1910400]
- European Regional Development Fund [CZ.02.1.01/0.0/0.0/16_019/0000845]
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This paper introduces a fast, easy, and non-invasive method for analyzing three boar taint compounds in boar saliva samples. The method shows good reproducibility, accuracy, and precision, and does not require derivatization.
Boar taint compounds influence the sexual behavioral responses of sows and stimulate their reproduction. This paper reports a fast, easier, and a non-invasive analytical method for the analysis of three boar taint compounds in boar' saliva samples: androstenone, androsten-3 alpha-ol, and androsten-38-ol. This method was developed and validated based on solid-phase extraction (SPE) and multidimensional gas chromatography-mass spectrometry (MDGC-MS). All the compounds were detected without derivatization. This method affords good reproducibility (4%-8%), accuracy (80%-105%), precision (5.5%-9.1%), linearity (R2 = 0.98-0.99), and lower limits of quantitation (LLOQ) (0.1-0.2 mu g/L). Although the presence of these compounds in saliva has been known for a long time, no simple and easy analytical method has been developed.
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