Journal
ANALYTICA CHIMICA ACTA
Volume 1235, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.aca.2022.340536
Keywords
Dual -signal visual detection; Ag; Au nanoparticle; Organophosphorus pesticide; Localized surface plasmon resonance; Fluorescence
Categories
Funding
- Jiangsu Planned Projects for Post- doctoral Research Funds
- China Postdoctoral Science Foundation
- [2020Z302]
- [2021T140333]
- [2020M681647]
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In this work, a dual-signal visual biosensor was designed using DNA functionalized Ag/Au bimetallic nanoparticles as a multifunctional nanoprobe for the detection of organophosphorus pesticides. The biosensor showed high selectivity and sensitivity through the inhibition of enzyme-induced H2O2 generation by the pesticides. The nanoprobe exhibited significant localized surface plasmon resonance and fluorescence signal responses, allowing for simultaneous quantitative measurement of the pesticides.
In this work, a dual-signal visual biosensor was designed for organophosphorus pesticides (OPs) detection using DNA functionalized Ag/Au bimetallic nanoparticles (Ag/Au NPs) as multifunctional nanoprobe. The dual-signal detection strategy was based on the inhibition of enzyme-induced H2O2 generation by OPs in the detection solution containing acetylcholinesterase (AChE), choline oxidase (CHO), acetylcholine (ACh) and nanoprobe. H2O2 produced by enzyme-catalyzed reaction could trigger the etching of Ag and dissociation of carboxy-fluorescein (FAM)-labeled aptamer from the nanoprobe, resulting in significant localized surface plasmon resonance (LPRR) and fluorescence (FL) signal responses. In the presence of OPs, AChE activity was inhibited to disrupt the enzymatic generation of H2O2, which allowed to simultaneous quantitative measure OPs through the LSPR peak shifts and FL intensity variations of the nanoprobe. The LSPR/FL dual-signal biosensor showed great selectivity and sensitivity for OPs detection. In addition, two distinct colour changes were visually observed to match the LSPR/FL spectra signal responses, which was a feasible means for visual analysis of OPs. Consequently, the work provided a dual-signal visual biosensor via the combination of multifunctional nanoprobe, and had significant potential to monitor pesticide residue with high anti-interference capability and detection accuracy.
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