Selection and electrochemical-sensor application of an DNA-aptamer for methyl parathion detection

An aptamer-based electrochemical sensor for methyl parathion (MP) detection is herein reported. The modified magnetic beads-systematic evolution of ligands by enrichment (MB-SELEX) was used to select the MP aptamer. After 14 rounds of selection, the aptamer (MPapta-6) with high affinity for MP was obtained, and its dissociation constant (Kd) was 39.66 +/- 14.73 mu M. Using the MPapta-6, the ultra-sensitive electrochemical sensor based on PLL-BP and AuNPs was constructed. The linear range of MP was 1-105 pM and detection limit (LOD) was as low as 0.49 pM. In addition, the application of the sensor in water samples was verified, and the recovery rate was 96.6%-103.5%. The results from this study showed that this strategy could be applied in practical detection.

Automated summary

An aptamer-based electrochemical sensor was developed for detecting methyl parathion (MP). The MP aptamer (MPapta-6) with high affinity for MP was obtained using the modified magnetic beads-systematic evolution of ligands by enrichment (MB-SELEX). The constructed sensor showed a linear range of 1-105 pM with a low detection limit (LOD) of 0.49 pM. The sensor was successfully applied to detect MP in water samples with a recovery rate of 96.6%-103.5%.