Journal
JOURNAL OF NEUROINFLAMMATION
Volume 13, Issue -, Pages -Publisher
BIOMED CENTRAL LTD
DOI: 10.1186/s12974-016-0694-4
Keywords
alpha-Synuclein; Experimental autoimmune encephalomyelitis; Neuroinflammation; Multiple sclerosis
Categories
Funding
- Interdisciplinary Center for Clinical Research Erlangen (IZKF) [E18, E24]
- German Parkinson Association
- Deutsche Forschungsgemeinschaft (DFG)
- Austrian Science Fund (FWF) [E24] Funding Source: Austrian Science Fund (FWF)
Ask authors/readers for more resources
Background: Increased alpha-synuclein immunoreactivity has been associated with inflammatory activity in multiple sclerosis (MS) lesions, but the function of alpha-synuclein in neuroinflammation remains unknown. The aim of this study was to examine the role of alpha-synuclein in immunological processes in murine experimental autoimmune encephalomyelitis (EAE) as a model of MS. Findings: We studied EAE in wildtype (aSyn(+/+)) and alpha-synuclein knockout (aSyn(-/-)) mice on a C57BL/6N background. In the spleen and spinal cord of aSyn(+/+) mice, we observed a gradual reduction of alpha-synuclein expression during EAE, starting already in the pre-symptomatic disease phase. Compared to aSyn(+/+) mice, aSyn(-/-) mice showed an earlier onset of symptoms but no differences in symptom severity at the peak of disease. Earlier symptom onset was accompanied by increased spinal cord infiltration of CD4(+) T cells, predominantly of interferon-gamma-producing T helper 1 (Th1) cells, and reduced infiltration of regulatory T cells, whereas antigen-presenting cells were unaltered. Pre-symptomatically, aSyn(-/-) mice exhibited hyperproliferative CD4(+) splenocytes consistent with increased splenic interleukin-2 mRNA expression, resulting in increased numbers of Th1 cells in the spleen at the onset of symptoms. Conclusions: Our findings indicate a functional role of alpha-synuclein in early EAE by increasing Th1 cell-mediated immune response.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available