Dynamically Reconstructed Collagen Fibers for Transmitting Mechanical Signals to Assist Macrophages Tracing Breast Cancer Cells

Constructing proper in vitro tumor immune microenvironment (TIME) is important for cancer immune-therapy studies, while the selection of biomaterials is critical. As innate immune cells, macrophages can target and kill cancer cells in vivo at the early stage of tumor development. However, this targeting phenomenon has not been observed in vitro. Herein, a quasi-3D in vitro cell culture model is constructed to mimic TIME by integrating hydrogel collagen as extracellular matrix for cells. In the collagen-based quasi-3D in vitro system, for the first time, it is found that macrophages can be attracted toward cancer cells along the dynamically reconstructed collagen fibers. By combining traction force microscopy and customized micro-manipulator system, it is revealed that the collagen matrix-transmitted tensile force signaling precisely guides the migration of macrophages toward cancer cells. The mechano-responsiveness mechanism is related to the activation of mechanosensitive ion channels, and the induced local increase of calcium signal, which is proved to enhance the F-actin assembly and to guide the cell migration. This novel mechanism advances the understanding of the role of collagen fibers in mechanotaxis of macrophages. Taken together, it has great potential for assisting biomaterial designs in developing new drug-screening models and clinical strategies for cancer immune-therapy.

Automated summary

Constructing a proper in vitro tumor immune microenvironment (TIME) is crucial for cancer immune-therapy studies, and the selection of biomaterials is critical. This study constructs a quasi-3D in vitro cell culture model by integrating hydrogel collagen as extracellular matrix to mimic TIME. The research reveals a novel mechanism where the collagen matrix-transmitted tensile force guides the migration of macrophages toward cancer cells, providing a better understanding of the role of collagen fibers in mechanotaxis of macrophages. This has great potential for developing new drug-screening models and clinical strategies for cancer immune-therapy.