Intracellular pH Sensor Based on Heteroleptic Bis-Cyclometalated Iridium(III) Complex Embedded into Block-Copolymer Nanospecies: Application in Phosphorescence Lifetime Imaging Microscopy

Herein, a novel pH-responsive phosphorescent probe based on cyclometalated iridium(III) complex is reported. To prevent oxygen quenching of phosphorescence and to improve the probe biocompatibility, the complex is covalently conjugated with a water-soluble block-copolymer that also increases its pH sensitivity. The resulting polymeric nanoprobe demonstrates a strong response of the phosphorescence lifetime onto pH variations in physiological range. Cellular experiments with Chinese hamster ovary (CHO-K1) cells show the predominant internalization of the probe in acidified cell compartments, endosomes and lysosomes. The analysis of phosphorescence lifetime imaging microscopy data confirms applicability of the sensor for monitoring of intra- and extracellular pH in cell cultures.

Automated summary

A novel pH-responsive phosphorescent probe based on cyclometalated iridium(III) complex is reported, which is covalently conjugated with a water-soluble block-copolymer to prevent oxygen quenching and improve biocompatibility. The resulting polymeric nanoprobe shows a strong response of phosphorescence lifetime to pH variations in the physiological range. Cellular experiments confirm the applicability of this sensor for monitoring intra- and extracellular pH in cell cultures.