4.6 Article

Uncovering a Subtype of Microviridins via the Biosynthesis Study of FR901451

Related references

Note: Only part of the references are listed.
Article Biochemistry & Molecular Biology

Structural and biochemical studies of an iterative ribosomal peptide macrocyclase

Gengnan Li et al.

Summary: This study reports the structure and biochemical characteristics of AMdnB involved in microviridin biosynthesis from Anabaena sp. PCC 7120, shedding light on the iterative macrocyclization mechanism. AMdnB can introduce multiple macrocyclizations on a precursor peptide in a distributive mode of catalysis. The results provide insight into the general mechanism of iterative enzymes in peptide biosynthetic pathways.

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS (2022)

Article Multidisciplinary Sciences

Correlational networking guides the discovery of unclustered lanthipeptide protease-encoding genes

Dan Xue et al.

Summary: Many natural product biosynthetic gene clusters lack necessary genes and rely on enzymes encoded elsewhere in the genome for biosynthesis. In this study, the authors establish a global correlation network to bridge the gap between lanthipeptide precursors and hidden proteases. Through analysis of bacterial genomes, they identify 5209 correlations between precursors and hidden proteases, with 91 prioritized. They also discover a new family of lanthipeptide proteases and demonstrate their involvement in the synthesis of class III lanthipeptides.

NATURE COMMUNICATIONS (2022)

Article Multidisciplinary Sciences

Radical SAM-dependent ether crosslink in daropeptide biosynthesis

Sijia Guo et al.

Summary: Darobactin is a potent peptide with a unique bicyclic scaffold that shows strong activity against Gram-negative bacteria. The researchers identify the enzyme DarE as responsible for forming the bicyclic scaffold and propose the name daropeptide for this growing class of enzymes. Genome mining analysis reveals the diversity of darobactin-like biosynthetic gene clusters.

NATURE COMMUNICATIONS (2022)

Article Microbiology

Compendium of specialized metabolite biosynthetic diversity encoded in bacterial genomes

Athina Gavriilidou et al.

Summary: By analyzing a large number of bacterial genomes and metagenome assembled genomes (MAGs), it was found that only about 3% of the potential natural products encoded in bacterial genomes have been characterized. The study also identified the genus level as an appropriate taxonomic rank for comparing the biosynthetic diversity of secondary metabolites, with Streptomyces bacteria encoding the largest diversity. Additionally, less-studied taxa such as Weeksellaceae, Myxococcaceae, Pleurocapsa, and Nostocaceae were found to have the potential to produce diverse secondary metabolites that require further investigation.

NATURE MICROBIOLOGY (2022)

Article Multidisciplinary Sciences

Biosynthetic potential of the global ocean microbiome

Lucas Paoli et al.

Summary: Natural microbial communities are diverse and offer great potential for the discovery of enzymes and biochemical compounds. However, studying this diversity and assigning the synthesis of compounds to their hosts is challenging. In this study, we integrated microbial genomes from various sources and discovered thousands of new biosynthetic gene clusters, including in previously unsuspected phylogenetic groups. We identified a lineage rich in biosynthetic gene clusters and characterized the structures and enzymology of bioactive compounds. This research demonstrates the value of microbiomics-driven strategies in exploring previously undescribed enzymes and natural products.

NATURE (2022)

Review Biochemistry & Molecular Biology

Bioactive natural products from Bacteroidetes

Stephan Brinkmann et al.

Summary: This article discusses the diverse range of natural products produced by bacteria representing the Bacteroidetes phylum, including polyketides, peptides, and lactams. It also provides an overview of the unique aspects of the discovered bioactive compounds and their corresponding biosynthetic pathways, highlighting the Bacteroidetes as a natural product reservoir.

NATURAL PRODUCT REPORTS (2022)

Article Biochemistry & Molecular Biology

Bioinformatics-Guided Expansion and Discovery of Graspetides

Sangeetha Ramesh et al.

Summary: Graspetides are a class of ribosomally synthesized and post-translationally modified peptide natural products that are formed through ATP-grasp ligase-dependent formation of macrolactones/macrolactams. These modifications are derived from different amino acid residues and characterized graspetides include serine protease inhibitors.

ACS CHEMICAL BIOLOGY (2021)

Review Chemistry, Medicinal

Current Knowledge on Microviridin from Cyanobacteria

Samuel Cavalcante do Amaral et al.

Summary: Microviridins, a class of ribosomal peptides derived from cyanobacteria, are potent protease inhibitors with potential applications in drug development and mosquito control. They also play a key ecological role in defending cyanobacteria against microcrustaceans. This review aims to systematically identify the key characteristics of microviridins, including their chemical structure, biosynthesis, as well as their biotechnological and ecological significance.

MARINE DRUGS (2021)

Review Biochemistry & Molecular Biology

Digestive Inflammation: Role of Proteolytic Dysregulation

Vincent Mariaule et al.

Summary: Proteolytic imbalance is often linked to diseases, especially in digestive inflammation. Serine proteases and matrix metalloproteases play crucial roles in inflammatory responses, but the imbalance between these enzymes and their inhibitors could contribute to disease pathogenesis.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

Article Biochemistry & Molecular Biology

antiSMASH 6.0: improving cluster detection and comparison capabilities

Kai Blin et al.

Summary: antiSMASH, a tool for detecting biosynthetic gene clusters in microorganisms, has been updated to version 6, which increases the supported cluster types, displays the structure of multi-modular BGCs, adds a new comparison algorithm, allows integration of results from other prediction tools, and improves detection of tailoring enzymes in RiPP clusters.

NUCLEIC ACIDS RESEARCH (2021)

Article Chemistry, Multidisciplinary

Structural Basis for a Dual Function ATP Grasp Ligase That Installs Single and Bicyclic ω-Ester Macrocycles in a New Multicore RiPP Natural Product

Gengxiang Zhao et al.

Summary: Chryseoviridin, a newly discovered multicore RiPP, contains four consecutive five- or six-residue macrocycles ending with a microviridin-like core. The crystal structure reveals a significant conformational change in the B-domain of the ATP-grasp ligase CdnC upon substrate binding, providing insights into omega-ester bond formation in RiPP biosynthesis. Experimental validation confirms a model where macrocyclization occurs in the N- to C-terminal direction for core peptides containing multiple Thr/Ser-to-Asp macrocycles.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2021)

Article Biochemistry & Molecular Biology

Heterologous production of new protease inhibitory peptide marinostatin E

Kohta Unno et al.

Summary: In this study, a new analogous peptide named marinostatin E was successfully produced using Escherichia coli, exhibiting inhibitory activities against chymotrypsin and subtilisin. The structure of marinostatin E was determined through chemical treatments and tandem mass spectrometry experiments.

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY (2021)

Review Biochemistry & Molecular Biology

New developments in RiPP discovery, enzymology and engineering

Manuel Montalban-Lopez et al.

Summary: This article reviews the research progress of ribosomally-synthesized and post-translationally modified peptides (RiPPs) as of June 2020, including the discovery of new RiPP classes, deeper understanding of the mechanisms for the installation of post-translational modifications, and the mechanisms by which enzymes recognize leader peptides. Additionally, genome mining tools and strategies for RiPP engineering are also discussed in the review.

NATURAL PRODUCT REPORTS (2021)

Article Plant Sciences

Microviridin 1777: A Toxic Chymotrypsin Inhibitor Discovered by a Metabologenomic Approach

Simon Sieber et al.

JOURNAL OF NATURAL PRODUCTS (2020)

Article Chemistry, Multidisciplinary

Genome Mining Reveals High Topological Diversity of ω-Ester-Containing Peptides and Divergent Evolution of ATP-Grasp Macrocyclases

Hyunbin Lee et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2020)

Article Biotechnology & Applied Microbiology

Heterologous expression of a cryptic gene cluster from Grimontia marina affords a novel tricyclic peptide grimoviridin

Kohta Unno et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2020)

Review Oncology

Cell surface-anchored serine proteases in cancer progression and metastasis

Carly E. Martin et al.

CANCER AND METASTASIS REVIEWS (2019)

Article Chemistry, Multidisciplinary

Leader Peptide-Free In Vitro Reconstitution of Microviridin Biosynthesis Enables Design of Synthetic Protease-Targeted Libraries

Emmanuel Reyna-Gonzalez et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2016)

Article Biochemistry & Molecular Biology

Structural basis for precursor protein-directed ribosomal peptide macrocyclization

Kunhua Li et al.

NATURE CHEMICAL BIOLOGY (2016)

Article Multidisciplinary Sciences

Crystal structures of a polypeptide processing and secretion transporter

David Yin-wei Lin et al.

NATURE (2015)

Article Biochemistry & Molecular Biology

Leader Peptide and a Membrane Protein Scaffold Guide the Biosynthesis of the Tricyclic Peptide Microviridin

Annika R. Weiz et al.

CHEMISTRY & BIOLOGY (2011)

Review Genetics & Heredity

Congenital neutropenia: diagnosis, molecular bases and patient management

Jean Donadieu et al.

ORPHANET JOURNAL OF RARE DISEASES (2011)

Article Biochemistry & Molecular Biology

Substrate Specificity and Scope of MvdD, a GRASP-like Ligase from the Microviridin Biosynthetic Gene Cluster

Benjamin Philmus et al.

ACS CHEMICAL BIOLOGY (2009)

Article Chemistry, Multidisciplinary

Ribosomal synthesis of tricyclic depsipeptides in bloom-forming cyanobacteria

Nadine Ziemert et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2008)

Article Biochemistry & Molecular Biology

Post-translational Modification in Microviridin Biosynthesis

Benjamin Philmus et al.

CHEMBIOCHEM (2008)

Article Chemistry, Organic

New microviridins from a water bloom of the cyanobacterium Microcystis aeruginosa

Vered Reshef et al.

TETRAHEDRON (2006)

Article Biochemistry & Molecular Biology

Solution structure of marinostatin, a natural ester-linked protein protease inhibitor

K Kanaori et al.

BIOCHEMISTRY (2005)

Article Biochemistry & Molecular Biology

Isolation, characterization, and quantitative analysis of microviridin J, a new Microcystis metabolite toxic to Daphnia

T Rohrlack et al.

JOURNAL OF CHEMICAL ECOLOGY (2003)

Article Microbiology

ABC transporters: physiology, structure and mechanism - an overview

CF Higgins

RESEARCH IN MICROBIOLOGY (2001)