Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 14, Issue 50, Pages 55320-55331Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.2c11946
Keywords
self-assembly; liposomes; membrane activity; spiropyran; peptide bilayer; lipid bilaye r fusion
Funding
- Hungarian Momentum Program [LP2016-2]
- National Competitiveness and Excellence Program [NVKP_16-1-2016-0007, BIONANO_GINOP2.3.2-15-2016-00017]
- National Research, Development and Innovation Office, Hungary [TKP2021EGA-31, 2020-1.1.2-PIACI-KFI-2020-00021, 2019-2.1.11TE'T-2019-00091, KKP_22, 144180,, K131594]
- Eotvos Lorand Research Network [SA-87/2021, KEP-5/2021]
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Self-assembled peptide nanostructures with stimuli-responsive features have the potential as functional materials. In this study, a short membrane anchor protein motif was coupled with a spiropyran photoswitch to achieve controllable interactions with lipid membranes. Photo-isomerization triggered rearrangements within the peptide bilayer, resulting in significant changes in their membrane-binding properties.
Self-assembled peptide nanostructures with stimuli-responsive features are promising as functional materials. Despite extensive research efforts, water-soluble supramolecular constructs that can interact with lipid membranes in a controllable way are still challenging to achieve. Here, we have employed a short membrane anchor protein motif (GLFD) and coupled it to a spiropyran photoswitch. Under physiological conditions, these conjugates assemble into similar to 3.5 nm thick, foil-like peptide bilayer morphologies. Photo-isomerization from the closed spiro (SP) form to the open merocyanine (MC) form of the photoswitch triggers rearrangements within the foils. This results in substantial changes in their membrane-binding properties, which also varies sensitively to lipid composition, ranging from reversible nanofoil reformation to stepwise membrane adsorption. The formed peptide layers in the assembly are also able to attach to various liposomes with different surface charges, enabling the fusion of their lipid bilayers. Here, SP-to-MC conversion can be used both to trigger and to modulate the liposome fusion efficiency.
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