Journal
FORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES
Volume 8, Issue 1, Pages 156-158Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.fsigss.2022.10.019
Keywords
Tissue homogenization; Burnt bone; Forensic casework
Categories
Funding
- Forensic Genetics Research Unit, Ratchadapiseksompotch Fund, Department of Forensic Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok Thailand
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This study utilized a new technique for bone homogenization in forensic DNA analysis, which proved to be time-saving, efficient, and suitable for high-throughput productivity.
Sample disruption was a necessary step for DNA isolation. Bone and teeth were useful biological sources particular in human remains and advance decomposed bodies. The compact bone and teeth required several preparation steps prior to analyzing process. However, the methods in standard protocol were laborious and time consuming. An alternating pulverization, bead beating homogenizer, was purposed in its effectiveness for forensic casework. (1) Here, we applied this technique to the burnt cracked bone and tooth that recovered from house fire for forensic DNA analysis. After cleansed an external surface, the eight multidirectional motion tissue homogenizer, Precellys & REG; evolution, was utilized to pulverize bone and tooth followed by a DNA extraction and amplification. For detection with a capillary electrophoresis, full profiles of autosomal STRs and Y-chromosomal STRs were recovered from tooth sample but the partial profile STR was demonstrated in bone sample. The new technique in bone homogenization was less time consuming (around 30 s), less exposure to chemical agents (no need of liquid nitrogen), high efficiency, with high-throughput productivity.
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