Journal
JOURNAL OF NANOPARTICLE RESEARCH
Volume 18, Issue 2, Pages -Publisher
SPRINGER
DOI: 10.1007/s11051-016-3337-x
Keywords
Magnetic nanoparticles; Lipase immobilization; Dendritic modification
Categories
Funding
- National Natural Science Foundation of China [21374045, 21074049]
- Fundamental Research Funds for the Central Universities [lzujbky-2014-186]
Ask authors/readers for more resources
This paper reported an immobilization of Candida rugosa lipase (CRL) onto PAMAM-dendrimer-grafted magnetic nanoparticles synthesized by a modified solvothermal reduction method. The dendritic magnetic nanoparticles were amply characterized by several instrumental measurements, and the CRL was covalently anchored on the three generation supports with glutaraldehyde as coupling reagent. The amount of immobilized enzyme was up to 150 mg/g support and the factors related with the enzyme activity were investigated. The immobilization of lipase improved their performance in wider ranges of pH and temperature. The immobilized lipase exhibited excellent thermal stability and reusability in comparison with free enzyme and can be reused 10 cycles with the enzymatic activity remained above 90 %. The properties of lipase improved obviously after being immobilized on the dendritic supports. The inactive immobilized lipase could be regenerated with glutaraldehyde and Cu2+, respectively. This synthetic strategy was facile and eco-friendly for applications in lipase immobilization.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available