Generation of self-assembling cardiac organoids using hiPSC-derived cardiomyocytes

The combination of the human induced pluripotent stem cell (hiPSC) and organoid technology enables the generation of human 3D culture systems, providing the opportunity to model human tissue-like structures in vitro. This protocol offers the details to generate and characterize self-assembling 3D cardiac organoids in a controlled and efficient manner based on hiPSC-derived cardiomyocytes. Cardiac organoids can be used as 3D-based assay systems and offer a wide range of applications in pharmacological and toxicological research as well as an alternative to animal experiments.

Automated summary

The combination of hiPSC and organoid technology allows the generation of 3D human culture systems, providing a means to model human tissue-like structures in vitro. This protocol provides detailed instructions to efficiently generate and characterize self-assembling 3D cardiac organoids using hiPSC-derived cardiomyocytes. Cardiac organoids can serve as 3D-based assay systems with various applications in pharmacological and toxicological research, offering an alternative to animal experiments.