Journal
HELIYON
Volume 8, Issue 10, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.heliyon.2022.e11013
Keywords
Caesalpinia sinensis; Proliferation; Cell cycle; Migration; Invasion
Categories
Funding
- National Natural Science Foundation of China [21768005, 31760095, 81960639]
Ask authors/readers for more resources
The compound 3-desoxysulforaphane (3-DSC) isolated from Caesalpinia sinensis showed antitumor activity by inhibiting cell proliferation, migration, and invasion in HeLa and PC3 cells through modulation of multiple signaling pathways.
To study the antitumor activity of compound 3-desoxysulforaphane (3-DSC) isolated from Caesalpinia sinensis, SRB assay, clone formation assay, flow cytometric cell cycle assay, scratch assay, transwell assay, and molecular docking were used to investigate the inhibitory effect of 3-DSC on HeLa and PC3 cells. The results showed that 3-DSC inhibited the cell migration and invasion by down-regulating expression of N-cadherin, Vimentin, MMP-2, and MMP-9 in HeLa and PC3 cells; It also inhibits cell proliferation by promoting the expression of CDK1 (cyclin-dependent kinases 1) and CDK2 (cyclin-dependent kinases 2), which arrests the tumor cell cycle at G2 phase. 3-DSC inhibits phosphorylation of AKT and ERK and upregulates the expression of the tumor suppressor gene p53. Molecular docking results confirmed that 3-DSC could bind firmly to AKT. In conclusion, 3-DSC inhibited the proliferation, migration and invasion of HeLa and PC3 cells.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available