Eco-Friendly Biocontrol Strategies of Alternaria Phytopathogen Fungus: A Focus on Gene-Editing Techniques

Agricultural food production is greatly affected by postharvest diseases worldwide, such as the diseases caused by Alternaria species, which are very common in several crops. The management of fungal infections around the world largely relies on fungicides. In this context, the control of diseases such as early blight caused by Alternaria solani in potatoes and Alternaria linariae in tomatoes has mainly consisted of the application of fungicides, with negative impacts on the environment and human health. Recently, the application of 'omics' and gene editing through the CRISPR/Cas9 system and RNAi technologies demonstrated their effectiveness as emerging greener alternatives for controlling phytopathogenic fungi. Additionally, coatings based on essential oils and microbial antagonists suggest alternative strategies for controlling phytopathogenic fungi that are respectful of the environment. This review presents an exhaustive literature review focused on using greener alternatives to the traditional management of postharvest diseases associated with Alternaria species, such as inhibiting pathogenicity from their phytopathogenic genes using gene editing based on CRISPR/Cas9 and RNAi technologies. The review also presents coatings based on essential oils and microbial antagonists as greener strategies for Alternaria control. Biological processes of maximum efficiency can replace chemical methods for controlling phytopathogenic fungi, preserving healthy conditions in agricultural lands and ecosystems. This is possible with the rise of 'omic' technologies, the CRISPR/Cas9 tool, and RNAi technology. Greener control methods of Alternaria fungi can increase agricultural production, improving the economy and global health.

Automated summary

Postharvest diseases, especially those caused by Alternaria species, have a significant impact on agricultural food production worldwide. Traditional management of these diseases relies heavily on the use of fungicides, which have negative effects on the environment and human health. However, recent advancements in 'omics' technologies, gene editing using CRISPR/Cas9 system and RNAi technologies, as well as the development of coatings based on essential oils and microbial antagonists, offer promising greener alternatives for controlling phytopathogenic fungi.