Journal
JOURNAL OF MICROSCOPY
Volume 263, Issue 2, Pages 200-211Publisher
WILEY
DOI: 10.1111/jmi.12424
Keywords
Osmium impregnation; plant membranes; SBFSEM; selective staining
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Funding
- BBSRC [BB/L014122/1]
- ERACAPs/BBSRC [BB/M000168/1]
- BBSRC [BB/M000168/1, BB/D524567/1, BB/L014122/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/L014122/1, BB/M000168/1, BB/D524567/1] Funding Source: researchfish
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Serial block face imaging with the scanning electron microscope has been developed as an alternative to serial sectioning and transmission electron microscopy for the ultrastructural analysis of the three-dimensional organization of cells and tissues. An ultramicrotome within the microscope specimen chamber permits sectioning and imaging to a depth of many microns within resin-embedded specimens. The technology has only recently been adopted by plant microscopists and here we describe some specimen preparation procedures suitable for plant tissue, suggested microscope imaging parameters and discuss the software required for image reconstruction and analysis.
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