Journal
LIFE-BASEL
Volume 12, Issue 9, Pages -Publisher
MDPI
DOI: 10.3390/life12091309
Keywords
Chlorella; biomass; cultivation; flat-panel photobioreactor; microalgae
Categories
Funding
- Russian Science Foundation (RSF) [21-74-30003, 20-14-00280]
- Ministry of Science and Higher Education of the Russian Federation [122042700043-9, 121050500047-5]
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This paper presents the growth characteristics of the microalgae Chlorella sorokiniana IPPAS C-1 in a specially designed cultivation system. The results show a significant increase in biomass through three-stage scale-up cultivation. The key parameters of the photobioreactors are also discussed.
Flat-panel photobioreactors are effective systems for microalgae cultivation. This paper presents the growth characteristics of the microalgae Chlorella sorokiniana IPPAS C-1 as a result of three-stage scale-up cultivation in a specially designed cultivation system. First, C. sorokiniana was grown aseptically in 250 mL glass vessels; then, it was diluted and inoculated into a 5-liter flat-panel horizontal photobioreactor; and, at the last stage, the culture was diluted and inoculated into a 70-liter flat-panel vertical photobioreactor. In the presented cycle, the cultured biomass increased by 326 times in 13 days (from 0.6 to 195.6 g dw), with a final biomass concentration of 2.8 g dw L-1. The modes of semi-continuous cultivation were considered. The biomass harvest and dilution of the suspension were carried out either every day or every 3-4 days. For C. sorokiniana IPPAS C-1, a conversion coefficient of optical density values to dry biomass (g L-1) was refined through a factor of 0.33. The key parameters of the photobioreactors tested in this work are discussed.
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