Journal
BIOSENSORS-BASEL
Volume 12, Issue 9, Pages -Publisher
MDPI
DOI: 10.3390/bios12090671
Keywords
COVID-19 biomarkers; SARS-CoV-2; serum; immunoglobulin; ferritin; interferometric optical detection method
Funding
- Research and Innovation program of Universidad Politecnica de Madrid [VIMPACTO20MHB]
- Spanish Ministry of economy and competitiveness [TEC2017-84846-R]
- regional government of Madrid (Spain)
Ask authors/readers for more resources
In this study, highly multiplexed diagnostic KITs based on an Interferometric Optical Detection Method were developed to evaluate six COVID-19-related biomarkers. The results showed that specific immunoglobulins, ferritin, and C-reactive protein play important roles in evaluating COVID-19.
In the present work, highly multiplexed diagnostic KITs based on an Interferometric Optical Detection Method (IODM) were developed to evaluate six Coronavirus Disease 2019 (COVID-19)-related biomarkers. These biomarkers of COVID-19 were evaluated in 74 serum samples from severe, moderate, and mild patients with positive polymerase chain reaction (PCR), collected at the end of March 2020 in the Hospital Clinico San Carlos, in Madrid (Spain). The developed multiplexed diagnostic KITs were biofunctionalized to simultaneously measure different types of specific biomarkers involved in COVID-19. Thus, the serum samples were investigated by measuring the total specific Immunoglobulins (sIgT), specific Immunoglobulins G (sIgG), specific Immunoglobulins M (sIgM), specific Immunoglobulins A (sIgA), all of them against SARS-CoV-2, together with two biomarkers involved in inflammatory disorders, Ferritin (FER) and C Reactive Protein (CRP). To assess the results, a Multiple Linear Regression Model (MLRM) was carried out to study the influence of IgGs, IgMs, IgAs, FER, and CRP against the total sIgTs in these serum samples with a goodness of fit of 73.01% (Adjusted R-Squared).
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available