Journal
BIOSENSORS-BASEL
Volume 12, Issue 10, Pages -Publisher
MDPI
DOI: 10.3390/bios12100775
Keywords
fipronil; black phosphorus nanosheets; aptamers; Au nanoparticles; electrochemical biosensors
Funding
- National Natural Science Foundation of China [61871180, 61971187]
- Natural Science Foundation of Hunan Province of China [2022JJ30230, 2021JJ30213]
- Hunan Provincial Innovation Foundation for Postgraduate [CX20211074]
- State Key Laboratory of Oral Diseases [SKLOD2022OF05]
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A label-free fipronil aptasensor was developed using Polylysine-black phosphorus nanosheets and gold nanoparticles. This sensor provides a quantitative detection method for fipronil.
A label-free fipronil aptasensor was built based on Polylysine-black phosphorus nanosheets composition (PLL-BPNSs) and Au nanoparticles (AuNPs). A PLL-BP modified glassy carbon electrode (GCE) was fabricated by combining BP NSs and PLL, which included a considerable quantity of -NH2. Au nanoparticles (AuNPs) were placed onto the GCE, and PLL-BPNSs bonded to Au NPs firmly by assembling. The thiolated primers were then added and fixed using an S-Au bond, and competitive binding of the fipronil aptamer was utilized for fipronil quantitative assessment. The sensor's performance was evaluated using differential pulse voltammetry (DPV) method. The linear equation is Delta I (mu A) = 13.04 logC + 22.35, while linear correlation coefficient R-2 is 0.998, and detection limit is 74 pg/mL (0.17 nM) when the concentration of fipronil is 0.1 ng/mL-10 mu g/mL. This aptasensor can apply to quantitative detection of fipronil.
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