Journal
BIOSENSORS-BASEL
Volume 12, Issue 9, Pages -Publisher
MDPI
DOI: 10.3390/bios12090770
Keywords
naphthalimide; styryl dye; crown ether; click-reaction; mercury (II) cation; A549 cells; HEK293 cells; ratiometric probe; sensor; fluorescence imaging; resonance energy transfer; photoinduced electron transfer
Funding
- RSCF (Russian Scientific Foundation) [20-7310186]
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In this study, a Dyad compound NI-SP with 1,8-naphthalimide (NI) and styrylpyridine (SP) photoactive units was evaluated as a ratiometric fluorescent chemosensor for mercury (II) ions in living cells. The compound selectively responds to the presence of Hg2+ by enhancing the emission intensity of NI while the fluorescence of SP remains virtually unchanged. This allows for self-calibration of the optical response. The compound shows potential for the detection and quantitative analysis of intracellular Hg2+ ions.
Dyad compound NI-SP bearing 1,8-naphthalimide (NI) and styrylpyridine (SP) photoactive units, in which the N-phenylazadithia-15-crown-5 ether receptor is linked with the energy donor naphthalimide chromophore, has been evaluated as a ratiometric fluorescent chemosensor for mercury (II) ions in living cells. In an aqueous solution, NI-SP selectively responds to the presence of Hg2+ via the enhancement in the emission intensity of NI due to the inhibition of the photoinduced electron transfer from the receptor to the NI fragment. At the same time, the long wavelength fluorescence band of SP, arising as a result of resonance energy transfer from the excited NI unit, appears to be virtually unchanged upon Hg2+ binding. This allows self-calibration of the optical response. The observed spectral behavior is consistent with the formation of the (NI-SP)center dot Hg2+ complex (dissociation constant 0.13 +/- 0.04 mu M). Bio-imaging studies showed that the ratio of fluorescence intensity in the 440-510 nm spectral region to that in the 590-650 nm region increases from 1.1 to 2.8 when cells are exposed to an increasing concentration of mercury (II) ions, thus enabling the detection of intracellular Hg2+ ions and their quantitative analysis in the 0.04-1.65 mu M concentration range.
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