Biodegradation of Dibutyl Phthalate by the New Strain Acinetobacter baumannii DP-2

Optimizing the culture conditions of DBP degradation by bacteria and investigating its biodegradation pathways have a great importance to develop effective PAEs pollution control strategies. In this study, we investigated the cultivation condition optimization, degradation kinetics, and degradation pathways of a newly isolated dibutyl phthalate (DBP) degradation strain, which was isolated from activated sludge and identified as Acinetobacter baumannii DP-2 via morphological observation, biochemical identification, and 16S rDNA sequence analysis. The degradation conditions were optimized based on the results of single-factor experiments and response surface optimization experiments. The DBP degradation rate of Acinetobacter baumannii DP-2 reached up to 85.86% when the inoculation amount was 17.14%, the DBP concentration was 9.81 mg.L-1 and the NaCl concentration was 5 g.L-1. The GC-MS analysis results indicated that the intermediate metabolites of Acinetobacter baumannii DP-2 mainly consisted of DMP, MBP, PA, and benzoic acid derivatives, which confirmed the degradation pathway from DBP to PA under aerobic pathway and then to BA under anaerobic pathway. In summary, Acinetobacter baumannii DP-2 shows great potential for the degradation of DBP in contaminated soils.

Automated summary

This study optimized the culture conditions of a DBP degradation strain and investigated its degradation pathways. The results showed that Acinetobacter baumannii DP-2 had a DBP degradation rate of up to 85.86%, with the pathway being DBP -> PA -> BA. This is of great importance for the development of effective PAEs pollution control strategies.