Journal
FRONTIERS IN NUTRITION
Volume 9, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fnut.2022.966705
Keywords
glycoprotein; structural characterization; immunomodulatory; RAW 264; 7 murine macrophage cell; chickpea
Categories
Funding
- National Key R&D Program of China [2021YFD1600100]
- earmarked fund for the China Agriculture Research System [CARS-08-G21]
- Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization
- Science and Technology Research Projects of Chongqing Education Commission [KJQN202102705]
Ask authors/readers for more resources
This study characterized the structure and evaluated the immunomodulatory activity of a glycoprotein (CAG-1) isolated from chickpea. CAG-1 contained polysaccharide and protein components and stimulated the production of various immune markers in macrophages.
Chickpea (Cicer arietinum L.) is a well-known legume widely used as traditional medicine. This study aimed to characterize the structure and evaluate the immunomodulatory activity of one glycoprotein [crude chickpea glycoprotein-1 (CAG-1)] isolated from chickpea. CAG-1 was extracted with hot alkaline water and purified with DEAE-Sepharose Fast Flow and Superdex-200 column chromatography. CAG-1, with a molecular weight of 8,106 Da, contained 57.12% polysaccharide and 35.41% protein. The polysaccharide part was mainly composed of glucose (Glc). The protein part was connected mainly by aspartic (Asp) and glutamic (Glu). The results of nuclear magnetic resonance (NMR) analysis indicated the presence of alpha-d-Glcp-(1 -> 4)-alpha-d-Glcp-(1 -> 4)-alpha-d-Glcp-(1 -> . In addition, the sugar chains of the glycoprotein were not hydrolyzed under alkaline conditions, suggesting that the glycoprotein was N-glycosidic; thus, the sugar chain was linked to the protein chain by Asp. An immunological study showed that CAG-1 stimulated the production of nitric oxide (NO), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), and monocyte chemotactic protein 1 (MCP-1) in RAW 264.7 macrophages in a dose-dependent manner.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available