Journal
FRONTIERS IN NUTRITION
Volume 9, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fnut.2022.972569
Keywords
Lactobacillus paracasei ZFM54; Helicobacter pylori; gastritis; gastric microbiota; inflammation cytokines
Categories
Funding
- Chinese Academy of Engineering Academy-Locality Cooperation Project
- Natural Science Foundation of Zhejiang Province of China
- [2019-ZJ-JS-02]
- [LZ21C200001]
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The study showed that Lactobacillus paracasei ZFM 54 has the potential to protect against H. pylori infection by reducing inflammation and restoring the gastric microbiota.
Helicobacter pylori is the most prevalent pathogen causing chronic gastritis, gastroduodenal ulcers, and gastric tumors and is asymptomatically present in 50% of the world's population. This research is focused on investigating the effect of Lactobacillus paracasei ZFM 54 (CCTCC NO:2016667) on attenuating H. pylori-induced gastritis. H. pylori ZJC03 isolated from a patient with gastritis harbored the virulence genes of vacA and cagA and was highly resistant to metronidazole (MIC > 256 mu g/mL). In vitro analysis revealed that the potential anti-H. pylori characteristics of L. paracasei ZFM54 in terms of 65.57 +/- 1.87% survival rate in simulated gastric juices at a pH of 2.0, 69.00 +/- 2.73% auto-aggregation, 30.28 +/- 2.24% co-aggregation, 70.27 +/- 2.23% urease inhibition, and 57.89 +/- 1.27% radical scavenging. In H. pylori infectious mice, L. paracasei ZFM54 pre- and post-treatment reduced the levels of malondialdehyde in liver tissues to 0.71 +/- 0.04 nmol/mgprot (p < 0.05) and 0.70 +/- 0.06 nmol/mgprot (p < 0.05), respectively. Glutathione levels were increased to 1.78 +/- 0.02 mu mol/gprot (p < 0.05) and 1.76 +/- 0.52 mu mol/gprot (p < 0.05), respectively. L. paracasei ZFM54 significantly inhibited H. pylori-mediated inflammation observed in gastric mucosal repair and downregulated the mRNA expression of pro-inflammatory cytokines IFN-gamma, IL-1 beta, and IL-6 (p < 0.01). Importantly, L. paracasei ZFM54 increased Firmicutes and Actinobacteriota and decreased the relative abundance of bacterial taxa belonging to Campilobacterota and Proteobacteria. With the preventive and therapeutic administration of L. paracasei ZFM54, significant reductions in the average relative abundance of genera Helicobacter, Muribaculum, Staphylococcus, Lachnospiraceae_NK4A136_group, Prevotellaceae_UCG-001, Alloprevotella, and Oscillibacter were observed compared to infected mice. These findings suggest that L. paracasei ZFM 54 has the potential to protect against H. pylori infection by ameliorating inflammation and restoring the gastric microbiota.
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