Journal
ISCIENCE
Volume 25, Issue 10, Pages -Publisher
CELL PRESS
DOI: 10.1016/j.isci.2022.105080
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Funding
- Fondation Botnar (Switzerland)
- Swiss National Science Foundation [320030_ 173168, PCEGP3_181300, 31003A_176252]
- Clinical Research Priority Program of the University of Zurich Precision Medicine for Bacterial Infections
- Swiss National Science Foundation (SNF) [320030_173168, 31003A_176252, PCEGP3_181300] Funding Source: Swiss National Science Foundation (SNF)
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This study demonstrates that secondary electrospray ionization-high resolution mass spectrometry (SESI-HRMS) can be used as a diagnostic tool for rapid detection of bacterial infections. The detection of volatile organic compounds (VOCs) allows for species and strain level differentiation, and shows promise in clinical sample analysis.
Early detection of pathogenic bacteria is needed for rapid diagnostics allowing adequate and timely treatment of infections. In this study, we show that secondary electrospray ionization-high resolution mass spectrometry (SESI-HRMS) can be used as a diagnostic tool for rapid detection of bacterial infections as a supportive system for current state-of-the-art diagnostics. Volatile organic compounds (VOCs) produced by growing S. aureus or S. pneumoniae cultures on blood agar plates were detected within minutes and allowed for the distinction of these two bacteria on a species and even strain level within hours. Furthermore, we obtained a fingerprint of clinical patient samples within minutes of measurement and predominantly observed a separation of samples containing live bacteria compared to samples with no bacterial growth. Further development of this technique may reduce the time required for micro-biological diagnosis and should help to improve patient's tailored treatment.
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