Journal
JOURNAL OF FUNGI
Volume 8, Issue 10, Pages -Publisher
MDPI
DOI: 10.3390/jof8101028
Keywords
commensalism; gut; lipid; mitochondrial activity; reactive oxygen species; Candida albicans; Wor1
Categories
Funding
- Ministerio de Ciencia, Innovacion y Universidades [PID2021-122648NB-I00]
- CAM [PR38/21-32 ANTICIPA-CM]
- InGEMICS [B2017/BMD-3691]
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In this study, we investigated the role of the transcription factor Wor1 in commensalism by examining strains overexpressing this gene. We found that overexpression of WOR1 caused alterations in the lipid composition of fungal cells, increased sensitivity to membrane-disturbing agents and azole drugs, and changes in cellular physiology. These findings provide insights into the adaptation mechanisms of Candida albicans in the gastrointestinal tract.
Candida albicans is a commensal yeast that inhabits the gastrointestinal tract of humans; increased colonization of this yeast in this niche has implicated the master regulator of the white-opaque transition, Wor1, by mechanisms not completely understood. We have addressed the role that this transcription factor has on commensalism by the characterization of strains overexpressing this gene. We show that WOR1 overexpression causes an alteration of the total lipid content of the fungal cell and significantly alters the composition of structural and reserve molecular species lipids as determined by lipidomic analysis. These cells are hypersensitive to membrane-disturbing agents such as SDS, have increased tolerance to azoles, an augmented number of peroxisomes, and increased phospholipase activity. WOR1 overexpression also decreases mitochondrial activity and results in altered susceptibility to certain oxidants. All together, these changes reflect drastic alterations in the cellular physiology that facilitate adaptation to the gastrointestinal tract environment.
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