Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 125, Issue -, Pages 40-42Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2016.03.018
Keywords
Cell survival; Keratinocytes; HEPES; Candida albicans; Staphylococcus aureus
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This study assessed the effect of the buffers 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and 3(N-morpholino) propanesulfonic acid (MOPS) on keratinocyte cell viability and microbial growth. It was observed that RPMI buffered with HEPES, supplemented with L-glutamine and sodium bicarbonate, can be used as a more suitable medium to promote co-culture. (C) 2016 Published by Elsevier B.V.
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