Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 130, Issue -, Pages 23-26Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2016.08.020
Keywords
Next generation sequencing; Illumina; MiSeq; Library preparation; Angiostrongylus cantonensis; Cyclospora cayetanensis
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Illumina library preparation methods for ultra-low input amounts were compared using genomic DNA from two foodborne parasites (Angiostrongylus cantonensis and Cyclospora cayetanensis) as examples. The Ovation Ultralow method resulted in libraries with the highest concentration and produced quality sequencing data, even when the input DNA was in the picogram range. Published by Elsevier B.V.
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