Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 129, Issue -, Pages 78-80Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2016.08.005
Keywords
Metagenome; Human; Bead-beating
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Funding
- company Genetic Analysis AS, Oslo, Norway
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We evaluated bead-beating cell-lysis in analysing the human stool metagenome, since this is a key step. We observed that two different bead-beating instruments from the same producer gave a three-fold difference in the Bacteroidetes to Firmicutes ratio. This illustrates that bead-beating can have a major impact on downstream metagenome analyses. (C) 2016 Elsevier B.V. All rights reserved.
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