Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 127, Issue -, Pages 203-205Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2016.06.020
Keywords
CRISPR-Cas9; Sacchromyces cerevisiae; Metabolic engineering
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Funding
- CNPq (National Council for Scientific and Technological Development - Brazil) [239395/2012-3]
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CRISPR-Cas has become a powerful technique for genetic engineering of yeast Here, we present an improved version by using only one single plasmid expressing Cas9 and one or two guide-RNAs. A high gene deletion efficiency was achieved even with simultaneous recombination cloning of the plasmid and deletion in industrial strains. (C) 2016 Elsevier B.V. All rights reserved.
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