Application of CRISPR/Cas Systems in the Nucleic Acid Detection of Infectious Diseases

The CRISPR/Cas system is a protective adaptive immune system against attacks from foreign mobile genetic elements. Since the discovery of the excellent target-specific sequence recognition ability of the CRISPR/Cas system, the CRISPR/Cas system has shown excellent performance in the development of pathogen nucleic-acid-detection technology. In combination with various biosensing technologies, researchers have made many rapid, convenient, and feasible innovations in pathogen nucleic-acid-detection technology. With an in-depth understanding and development of the CRISPR/Cas system, it is no longer limited to CRISPR/Cas9, CRISPR/Cas12, and other systems that had been widely used in the past; other CRISPR/Cas families are designed for nucleic acid detection. We summarized the application of CRISPR/Cas-related technology in infectious-disease detection and its development in SARS-CoV-2 detection.

Automated summary

The CRISPR/Cas system is an immune system that defends against foreign genetic attacks with its excellent target-specific sequence recognition ability. It has shown great performance in the development of pathogen nucleic acid detection technology. Researchers have made rapid, convenient, and feasible innovations in pathogen nucleic acid detection technology by combining it with various biosensing technologies. The development of CRISPR/Cas system is not limited to CRISPR/Cas9 and CRISPR/Cas12, but other CRISPR/Cas families have been designed for nucleic acid detection.