Nematicidal Potential of Sulla (Hedysarum coronarium L.) against the Root-Knot Nematode Meloidogyne incognita

The content of nematicidal metabolites such as saponins, flavonoids and tannins in sulla (Hedysarum coronarium L.) suggests its potential nematicidal activity. In this study, the biocidal activity of 62.5-1000 mu g mL(-1) concentrations of flavonoid and tannin fractions from sulla was assessed in in vitro assays on the infective juveniles (J2) of the root-knot nematode (RKN) Meloidogyne incognita, while the suppressive effects of soil amendments with 10-40 g kg(-1) soil rates of sulla biomass were investigated on potted tomato infested by M. incognita. The content of total nitrogen, carbon, flavonoids, tannins and saponins of sulla experimental material was also determined. After a 96-h exposure, more than 80% of the M. incognita J2 were killed even by a 125 mu g mL(-1) concentration of the flavonoid extract, while mortality peaked at 89% only at the 1000 mu g mL(-1) concentration of the tannin solution. Soil incorporation with sulla biomass significantly reduced the M. incognita densities both on tomato roots and in the soil, compared to either the non-treated control and chemical treatment with Fluopyram. The data confirmed the nematicidal potential of sulla, mainly due to its content of flavonoids and tannins, suggesting its suitability as green manure or a soil amendment for sustainable RKN management.

Automated summary

This study investigated the biocidal activity of flavonoids and tannins in sulla, as well as the suppressive effects of sulla biomass on the root-knot nematode. Results showed that flavonoids and tannins in sulla have the potential to kill nematodes, and soil amendment with sulla biomass can effectively reduce nematode densities on tomato roots and in the soil.