The Effects of Temperature and Water on the Seed Germination and Seedling Development of Rapeseed (Brassica napus L.)

The seed germination and seedling growth of rapeseed are crucial stages in plant life, especially when facing abiotic stresses. In the present work, the effects of water and temperature on seed germination and seedling growth were investigated in a rapeseed crop (Brassica napus L.). The plants were examined under different temperature levels (5 degrees C, 10 degrees C, 15 degrees C, 20 degrees C, 25 degrees C, 30 degrees C, and 35 degrees C) and water levels (twenty-nine levels based on either one-milliliter intervals or as a percentage of the thousand-kernel weight (TKW)). Moreover, planting densities and antifungal application techniques were investigated in the study. The findings demonstrated substantial variations between all the growth parameters investigated at all the tested temperatures, and 20 degrees C was considered the optimum within a broad range of 15-25 degrees C. Water availability plays a significant role in germination, which can be initiated at 0.65 mL, corresponding to 500% of the TKW. The method of TKW is a more accurate aspect of water application because of the consideration of the seed weight and size. The optimal water range for the accumulation of dry weight, 3.85-5.9 mL (2900-4400% of TKW), was greater than that required for seedling growth, 1.45-3.05 mL (1100-2300% of TKW). Twenty to twenty-five seeds per 9 cm Petri dish exhibited the most outstanding values compared to the others, which provides an advantage in breeding programs, especially when there are seed limitations. Seed priming is a more effective antifungal application strategy. These data can be incorporated into future rapeseed germination in vitro studies, breeding programs, and sowing date predictions.

Automated summary

This study investigated the effects of different temperature and water conditions on rapeseed seed germination and seedling growth. The findings showed that 20 degrees Celsius was the optimum temperature for seeds, water availability played a significant role in germination, and seed quantity and priming method had an impact on seedling growth.