Journal
FOODS
Volume 11, Issue 19, Pages -Publisher
MDPI
DOI: 10.3390/foods11192990
Keywords
Piper nigrum L.; anti-inflammatory; piperine; MAPK; NF-kappa B
Categories
Funding
- Finance science and technology project of Hainan Province [SQKY2022-004, NKY-JCKY-2020, ZDYF2020149]
- Key Laboratory of Agro-Products Processing [S2021KFKT-29]
- Basic scientific research projects of Hainan Academy of Agricultural Science in China [JBKYYWF-2020-16]
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This study investigates the molecular mechanism of PIPERIN's anti-inflammatory effect in RAW264.7 cells and finds that it may regulate key factors of the NF-kappa B and MAPK signaling pathways.
Piperine, an important natural product, has a good anti-inflammatory effect. However, few researchers have studied its mechanism in these pathways. The objective of this research was to evaluate the molecular mechanism underlying the anti-inflammatory responses of piperine in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. The purification and characterization of piperine from Piper nigrum L. were determined by HPLC, UPLC-Q-TOF-MS and H-1 NMR. Then, the anti-inflammatory activity was evaluated by a reagent test kit, ELISA kits, RT-PCR and Western blot experiments. The results suggested that piperine (90.65 +/- 0.46% purity) at a concentration of 10-20 mg/L attenuated the production of NO and ROS, downregulated the protein and mRNA expression levels of TNF-alpha, IL-1 beta and IL-6, and upregulated the protein and mRNA transcription levels of IL-10. Meanwhile, the Western blot results indicated that piperine could inhibit the phosphorylation levels of the ERK, JNK, p38 and p65 proteins. Our findings suggest that piperine is a potential anti-inflammatory substance, whose molecular mechanism may be to regulate the key factors of the NF-kappa B and MAPK signalling pathways.
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