4.7 Article

Determination of Acetamiprid Residues in Vegetables by Indirect Competitive Chemiluminescence Enzyme Immunoassay

Journal

FOODS
Volume 11, Issue 16, Pages -

Publisher

MDPI
DOI: 10.3390/foods11162507

Keywords

acetamiprid; chemiluminescence enzyme immunoassay; rapid detection; residue; vegetable

Funding

  1. Natural Science Foundation of Jiangsu Province [BK20180916]

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Acetamiprid (ACE) is a widely used chemical in vegetables to control pests, but its residues pose a threat to human health. To rapidly detect ACE residues in vegetables, researchers have developed an indirect competitive chemiluminescence enzyme immunoassay (ic-CLEIA), which is simple, sensitive, and accurate.
Acetamiprid (ACE) is widely used in various vegetables to control pests, resulting in residues and posing a threat to human health. For the rapid detection of ACE residues in vegetables, an indirect competitive chemiluminescence enzyme immunoassay (ic-CLEIA) was established. The optimized experimental parameters were as follows: the concentrations of coating antigen (ACE-BSA) and anti-ACE monoclonal antibody were 0.4 and 0.6 mu g/mL, respectively; the pre-incubation time of anti-ACE monoclonal antibody and ACE (sample) solution was 30 min; the dilution ratio of goat anti-mouse-HRP antibody was 1:2500; and the reaction time of chemiluminescence was 20 min. The half-maximum inhibition concentration (IC50), the detection range (IC10-IC90), and the detection limit (LOD, IC10) of the ic-CLEIA were 10.24, 0.70-96.31, and 0.70 ng/mL, respectively. The cross-reactivity rates of four neonicotinoid structural analogues (nitenpyram, thiacloprid, thiamethoxam, and clothianidin) were all less than 10%, showing good specificity. The average recovery rates in Chinese cabbage and cucumber were 82.7-112.2%, with the coefficient of variation (CV) lower than 9.19%, which was highly correlated with the results of high-performance liquid chromatography (HPLC). The established ic-CLEIA has the advantages of simple pretreatment and detection process, good sensitivity and accuracy, and can meet the needs of rapid screening of ACE residues in vegetables.

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