Journal
FOODS
Volume 11, Issue 20, Pages -Publisher
MDPI
DOI: 10.3390/foods11203220
Keywords
pyrene; benzo [a]pyrene; monoclonal antibody; enzyme-linked immunoassay; aquatic product
Categories
Funding
- Key Research and Development Program of Hubei Province (CN) [2020BBB079]
- National Natural Science Foundation of China [32072920]
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An indirect competitive enzyme-linked immunoassay (ic-ELISA) was established to detect PYR and BaP residues in living aquatic products for the first time, with high sensitivity and accuracy.
Polycyclic aromatic hydrocarbons (PAHs) are significant environmental and food pollutants that can cause cancer. In this work, a specific monoclonal antibody (mAb) to identify pyrene (PYR) and benzo [a]pyrene (BaP) was prepared, and an indirect competitive enzyme-linked immunoassay (ic-ELISA) was established to detect PYR and BaP residues in living aquatic products for the first time. The effects of complete antigens with different coupling ratios on the production of high-sensitivity mAb was explored. Under the optimal conditions, the IC50 value was 3.73 +/- 0.43 mu g/L (n = 5). The limits of detection (LODs) for PYR and BaP in fish, shrimp, and crab ranged from 0.43 to 0.98 mu g/L. The average recoveries of the spiked samples ranged from 81.5-101.9%, and the coefficient of variation (CV) was less than 11.7%. The validation of the HPLC-FLD method indicated that the ELISA method set up in this experiment provided a trustworthy tool for PAHs residues detection in aquatic products.
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