Journal
FOODS
Volume 11, Issue 18, Pages -Publisher
MDPI
DOI: 10.3390/foods11182817
Keywords
high-intensity ultrasound; sodium caseinate; physicochemical; structure; emulsion stability
Categories
Funding
- National Natural Science Foundation of China [32072243]
- Natural Science Foundation of Henan Province [222300420092]
- Scientific research preferential funding project for Oversea Scholar of Henan Province [2022038]
- Foundation for University Young Key Teachers of Henan Province [2019GGJS128]
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High-intensity ultrasound treatment reduced particle size and improved surface properties of SC, enhancing emulsion stability without affecting its secondary structure. Overall, ultrasounds have the potential to stabilize SC O/W emulsions under various stress conditions.
The effects of high-intensity ultrasound treatment (0, 3, 6, 9 min) on physicochemical and structural characteristics of SC and the storage, thermal and freeze-thaw stability of SC O/W emulsions were investigated. The results showed that ultrasound treatment reduced the particle size of SC, although there were no obvious changes in zeta potential, profiles and weights. Ultrasound treatment improved surface hydrophobicity and fluorescence intensity of SC and changed ultraviolet-visible (UV-Vis) spectroscopy but had no influence on the secondary structure of SC. This indicates that ultrasounds might destroy the tertiary structure but leave most of the integral secondary structure. A scanning electron microscope (SEM) also showed that ultrasound-treated SC presented small aggregates and a loose structure. The physicochemical and structural changes of SC benefited the ability of protein adsorbing oil droplets and emulsion stability. Under stresses such as storage, thermal and freeze-thawing, the oil droplets of treated emulsions were still uniform and stable, especially at 6 min and 9 min. Overall, the high-intensity ultrasounds made the SC present small aggregates and a loose structure improving the SC O/W emulsions stability under storage, thermal and freeze-thawing environment and have great potential to stabilize the SC prepared O/W emulsions.
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