Effect of Water Activity on Conidia Germination in Aspergillus flavus

In this study, we explored the mechanism underlying Aspergillus flavus conidia germination inhibited by decreased water activity. The impact of low water activity was analyzed at 4 h, 8 h and 12 h. Additionally, we demonstrated that low water activity affected cell shape and decreased cell sizes. Transcriptomics found numerous differentially expressed genes (DEGs) during the first 12 h of germination, with 654 DEGs observed among 4 h, 8 h and 12 h. In particular, more DEGs were detected at 8 h of germinating. Therefore, proteomics was performed at 8 h, and 209 differentially expressed proteins (DEPs) were speculated, with 94 up-regulated and 115 down-regulated. Combined analysis of KEGG of transcriptomics and proteomics demonstrated that the dominant pathways were nutrient metabolism and translation. We also found several DEGs and DEPs in the Mitogen Activated Protein Kinase (MAPK) pathway. Therefore, we concluded that low water activity inhibited conidia germination, causing unregular morphology. In addition, low water activity influenced expression of creA, TreB in carbohydrate metabolism, Clr4, RmtA in amino acid metabolism and RPL37, RPL3 in translation in Aspergillus flavus.

Automated summary

In this study, the mechanism of Aspergillus flavus conidia germination inhibition by decreased water activity was explored. The impact of low water activity on cell shape and size was analyzed. Transcriptomics and proteomics identified differentially expressed genes and proteins, with dominant pathways involved in nutrient metabolism and translation. Low water activity was found to inhibit conidia germination and influence the expression of genes related to carbohydrate and amino acid metabolism, as well as translation in Aspergillus flavus.