4.6 Article

Manufactured Nano-Objects Confer Viral Protection against Cucurbit Chlorotic Yellows Virus (CCYV) Infecting Nicotiana benthamiana

Journal

MICROORGANISMS
Volume 10, Issue 9, Pages -

Publisher

MDPI
DOI: 10.3390/microorganisms10091837

Keywords

C-60 based-NPs; cucurbit chlorotic yellows virus; foliar application

Categories

Funding

  1. Princess Nourah bint Abdulrahman University Researchers Supporting Project, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia [PNURSP2022R84]
  2. Deanship of Scientific Research at King Khalid University [R.G.P 1-100/43]
  3. iASK Grant

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Nanotechnology has emerged as a new tool to combat phytopathogens in agricultural crops. This study found that C-60 fullerenes can inhibit the replication and systemic movement of cucurbit chlorotic yellows virus (CCYV).
Nanotechnology has emerged as a new tool to combat phytopathogens in agricultural crops. Cucurbit chlorotic yellows virus (CCYV) mainly infects Solanaceae crops and causes significant crop losses. Nanomaterials (NMs) may have efficacy against plant viruses, but the mechanisms underlying complex nanomaterials-plant-virus interactions remain elusive. We challenged Nicotiana benthamiana plants with GFP-tagged CCYV and observed morphological, physiological, and molecular changes in response to 21-d foliar exposure to nanoscale Fe and Zn and C-60 fullerenes at 100 mg/L concentration for 21 days. We observed that in response to C-60 (100 mg/L) treatment, plants displayed a normal phenotype while the viral infection was not seen until 5 days post-inoculation. On the contrary, Fe and Zn were unable to suppress viral progression. The mRNA transcriptional analysis for GFP and viral coat protein revealed that the transcripts of both genes were 5-fold reduced in response to C-60 treatment. Evaluation of the chloroplast ultrastructure showed that NMs treatment maintained the normal chloroplast structure in the plants as compared to untreated plants. C-60 upregulated the defense-related phytohormones (abscisic acid and salicylic acid) by 42-43%. Our results demonstrate the protective function of carbon-based NMs, with suppression of CCYV symptoms via inhibition of viral replication and systemic movement.

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